Bile Salt Hydrolase Activity Screening and Resistance to the Toxicity of Bile Salt by Indigenous Lactobacillus Isolates of Pakistan; A Research Article

Abstract

Objective: In this study 20 Lactobacillus strains isolated from different sources were selected for an account of their high bile-salt hydrolase (BSH) activity, bile-salt resistance, and tolerance to the toxicity of bile salts. Method: Bile salt hydrolase and resistance to toxic effects of bile salts were checked by assay for Deconjugation of bile salts and by toxicity of conjugated bile salt assay Results: A total of 20 isolates were tested for hydrolase activity of conjugated bile salts sodium salt taurocholic acid and glycocholic acid while toxicity of bile salts was in MRS broth supplemented various concentrations of bile salts. Out of 20 strains 2 strains displayed the largest precipitation zones were selected for toxicity assay. . Conclusion: The present study suggested that the finally two indigenous isolated of Lactobacillus from Pakistan has an excellent hypocholesterolemic effects. They will be used as a Probiotics to prevent hypercholesterolemia from human health while the mechanisms of regulating serum cholesterol and the effect on serum cholesterol level in vivo needs further extensive investigations.