La perméabilité des globules rouges humains aux ions orthophosphates

Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-11-08 DOI:10.1016/0926-6585(66)90008-2

Josette Chedru, Pierre Cartier

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引用次数: 10

Abstract

The kinetics of the influx of PO₄³⁻ into erythrocytes reveal a first-order process, with a half-time of 140 min at 37°.

The exchange measured for short times of incubation (0–300 sec) revealed that the incorporation of ³²P begins immediately and increases linearly. Inorganic phosphate is the precursor of phosphate in organic fractions: the highest initial specific activity was constantly found in this inorganic fraction. Incorporation of labelled phosphate into ATP and ADP is not so rapid and phosphate esters exchange very slowly.

The flux through the membrane remains proportional to the extracellular concentration and is not affected by a ten-fold increase above physiological conditions. This lack of saturation effect and null value for the affinity constant, excludes any participation of an active site located in the membrane.

The kinetics of permeability are not modified by competitive anions (SO₄²⁻, AsO₄³⁻). The AsO₄³⁻ ions decreased the uptake slowly; however, this effect is an indirect consequence of the inhibition of glycolysis.

The above results are in accordance with a process of simple diffusion.

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人体红细胞对正磷酸盐离子的渗透性

PO43−流入红细胞的动力学显示为一级过程，在37°时的半衰期为140 min。在孵育短时间内(0-300秒)测量的交换表明，32P的掺入立即开始并线性增加。无机磷酸盐是有机组分中磷酸盐的前体:在无机组分中不断发现最高的初始比活性。标记的磷酸盐与ATP和ADP的结合不是那么快，磷酸酯交换非常缓慢。通过膜的通量仍然与细胞外浓度成正比，并且不受以上生理条件增加十倍的影响。这种饱和效应的缺乏和亲和常数的零值，排除了位于膜上的活性位点的任何参与。渗透性动力学不受竞争性阴离子(SO42−，AsO43−)的影响。AsO43−离子缓慢地降低了摄取;然而，这种作用是抑制糖酵解的间接结果。上述结果符合简单扩散过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis

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