A population genetics approach for the study of fluridone resistance in hydrilla

Abstract

Fluridone-resistant hydrilla was first suspected in Florida in 1999 and was confirmed using molecular genetics techniques in 2003. Although the vast majority of species that evolve resistance to herbicides or other stressors do so through the genetic mutations that occur during sexual reproduction, all hydrilla in Florida is of the dioecious pistillate (“female”) biotype and all reproduction and spread is via vegetative means. The Hardy-Weinberg principle of constant allele frequencies (i.e., p + q = 1), used to predict allelic frequency shifts within populations due to selection, is based on a number of assumptions that are violated by species that reproduce asexually. In this paper, we address the assumptions of the model in the context of the clonally propagated species hydrilla and compare theoretical model predictions to the likely timeline of actual events that occurred in many bodies of water in Florida. The generational shifts in within-population allele frequencies from almost exclusively fluridone-susceptible to almost exclusively fluridoneresistant track well with the actual development of fluridone-resistant populations of hydrilla in Florida when considering fitness differences among fluridone resistance alleles after fluridone treatments. The present study illustrates how the Hardy-Weinberg principle of constant allele frequencies can be used as an exploratory tool to model resistance evolution in asexually reproducing species such as hydrilla.