Sequential Release of BMP-7 and VEGF from the PLGA/AK-Gelatin Composite Scaffolds

Gang Liu, W. Fan, X. Miao, Yin Xiao, David Good, M. Wei
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引用次数: 2

Abstract

Vascular endothelial growth factor (VEGF) and bone morphogenetic proteins (BMP-7) are key regulators of angiogenesis and osteogenesis during bone regeneration. The aim of this study was to investigate the possibility of realizing sequential release of the two growth factors using a novel composite scaffold. Poly(lactic-co-glycolic acid) (PLGA)-Akermanite (AK) microspheres were used to make the composite scaffold, which was then loaded with BMP-7, followed by embedding in a gelatin hydrogel matrix loaded with VEGF. The release profiles of the growth factors were studied and selected osteogenic related markers of bone marrow stromal cells (BMSCs) were analysed. It was shown that the composite scaffolds exhibited a fast initial burst release of VEGF within the first 3 days and a sustained slow release of BMP-7 over the full period of 20 days. The in vitro proliferation and differentiation of the BMSCs cultured in the osteogenic medium were enhanced by 1 to 2 times, resulting from the additionally and sequentially release of growth factors from the PLGA-AK/gelatin composite scaffolds.
BMP-7和VEGF在PLGA/ ak -明胶复合支架中的顺序释放
血管内皮生长因子(VEGF)和骨形态发生蛋白(BMP-7)是骨再生过程中血管生成和骨生成的关键调控因子。本研究的目的是探讨使用一种新型复合支架实现两种生长因子顺序释放的可能性。使用聚乳酸-羟基乙酸(PLGA)-Akermanite (AK)微球制作复合支架,然后负载BMP-7,然后包埋在负载VEGF的明胶水凝胶基质中。研究了生长因子的释放谱,分析了骨髓基质细胞成骨相关标志物。结果表明,复合支架在头3天内表现出快速的初始爆发性释放VEGF,在整个20天内表现出持续缓慢释放BMP-7。由于PLGA-AK/明胶复合支架中生长因子的附加和顺序释放,BMSCs在成骨培养基中培养的体外增殖和分化能力提高了1 ~ 2倍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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