LC-MS/MS METHOD FOR THE SIMULTANEOUS DETERMINATION OF CEFEPIME AND TAZOBACTAM IN DOG PLASMA

Drug Analytical Research Pub Date : 2018-09-14 DOI:10.22456/2527-2616.84357

K. Patil, Sukanta K. Naik, Vineet S. Zope, R. Chavan, R. Yeole

引用次数: 4

Abstract

A high performance liquid chromatography (HPLC) method with tandem mass spectrometric detection (MS/MS) has been developed and validated for the simultaneous quantification of cefepime and tazobactam in dog plasma. The method was developed on amide column with isocratic elution. The developed method is simple and economic in terms of sample preparation. The method is specific, sensitive, accurate, precise and robust. The method was successfully applied for pre-clinical pharmaco-kinetic studies in dogs. The Tmax was found to be 0.5 hr, the mean Cmax and AUC(0-12) displayed dose proportionate response.

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Lc-ms / ms法同时测定狗血浆中头孢吡肟和他唑巴坦的含量

建立了同时测定狗血浆中头孢吡肟和他唑巴坦含量的高效液相色谱-串联质谱检测(MS/MS)方法。该方法采用酰胺柱等密度洗脱法。该方法在样品制备方面简便、经济。该方法具有专属性强、灵敏度高、准确度高、精密度大、鲁棒性好等特点。该方法已成功应用于犬的临床前药物动力学研究。Tmax为0.5 hr，平均Cmax和AUC(0-12)呈剂量正比反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Drug Analytical Research

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