KINETICS STUDY OF THE GROWTH OF Lactobacillus bulgaricus FROM CAROB PODS SYRUP

Hariri Ahmed, Ouis Nawel, Bouhadi Djilali, Ould Yer Karima
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Abstract

The objective of this work consists in valuing the carob pods (very rich in sugars and in nourishing elements) as being a medium of culture for growth of Lactobacillus bulgaricus and comparison this natural syrup with MRS medium for lactic acid production curve. By its biochemical composition, the carob syrup is very rich in carbohydrates 16 g/L, protein 0.25 %, ash 0.8% including potassium (110 mg/100 mL of MF), sodium (80) and calcium (150). Kinetics study of growth of Lactobacillus bulgaricus in MRS and carob pods syrup showed that high biomass and lactic acid were obtained in the end of carob fermentations respectively (5.51 and 13.8) compared to MRS fermentation (4.57 and 10.9). This study clearly showed that carob pods have potential to be used as feedstock for lactic acid production by Lactobacillus bulgaricus using batch culture. Keyword: Carob pods syrup, fermentation, Lactobacillus bulgaricus.
角豆豆荚糖浆中保加利亚乳杆菌生长动力学研究
这项工作的目的是评估角豆荚(富含糖和营养元素)作为保加利亚乳杆菌生长的培养基,并将这种天然糖浆与MRS培养基进行乳酸生成曲线的比较。从其生化成分来看,角豆糖浆的碳水化合物含量非常丰富,为16克/升,蛋白质含量为0.25%,灰分含量为0.8%,其中钾(110毫克/100毫升MF),钠(80毫克)和钙(150毫克)。保加利亚乳杆菌在MRS和角豆豆荚糖浆中的生长动力学研究表明,与MRS发酵(4.57和10.9)相比,MRS发酵结束时分别获得了较高的生物量和乳酸(5.51和13.8)。本研究清楚地表明角豆荚有潜力作为保加利亚乳杆菌分批培养生产乳酸的原料。关键词:角豆荚糖浆,发酵,保加利亚乳杆菌
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