Polymorphic SSR marker identification for water use efficiency in groundnut (Arachis hypogea L.)parental lines

IF 0.8 4区生物学 Q3 AGRONOMY

Legume Research Pub Date : 2018-05-20 DOI:10.18805/LR-3980

C. Nandini, D. Savithramma, Pushpa Doddaraju, Pavan Kumar

引用次数: 2

Abstract

Groundnut is world important oilseed crop; productivity is low in the semi-arid regions due to frequent occurrence of drought. Identification of genotypes that have a greater ability to use limited available water is important to enhance productivity of the crop. Water Use Efficiency (WUE) is one such important trait, which increases yield under drought situation. Low level of DNA polymorphism has been detected in most of the laboratories due to allotetraploid nature. In the present study two parental lines of Recombinant Inbred line population NRCG12568 and NRCG12326 diverse for WUE related trait, such as Carbon Isotopic Discrimination (D13C), Specific Leaf Area (SLA) and SPAD chlorophyll meter reading were studied for polymorphism using 350 simple sequence repeat (SSR) markers. Out of these, only 119 (34%) markers showed polymorphism in NRCG12568 and NRCG12326. Detection of polymorphism opens up the possibility of use of these markers for QTL map development for D13C in groundnut.

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花生亲本水分利用效率的多态性SSR标记鉴定

花生是世界上重要的油料作物;半干旱地区干旱频繁，生产力低下。鉴定更有能力利用有限可用水分的基因型对提高作物生产力很重要。水分利用效率(WUE)是干旱条件下提高产量的重要指标之一。由于同种异体四倍体的性质，在大多数实验室中检测到低水平的DNA多态性。利用350个SSR标记对重组自交系群体NRCG12568和NRCG12326在WUE相关性状(D13C)、比叶面积(SLA)和SPAD叶绿素计读等方面的多态性进行了研究。其中，只有119个(34%)标记在NRCG12568和NRCG12326中存在多态性。多态性检测为利用这些标记开发花生D13C QTL图谱提供了可能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Legume Research AGRONOMY-

CiteScore

1.30

自引率

37.50%

发文量

256

审稿时长

6-12 weeks

期刊介绍： Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.