Behavior and possible function of Arabidopsis BES1/BZR1 homolog 2 in brassinosteroid signaling

IF 2.8 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Signaling & Behavior Pub Date : 2022-06-13 DOI:10.1080/15592324.2022.2084277

Yui Otani, Mika Kawanishi, Miyu Kamimura, A. Sasaki, Yasushi Nakamura, Takako Nakamura, S. Okamoto

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引用次数: 2

Abstract

ABSTRACT Two key transcription factors (TFs) in brassinosteroid (BR) signaling BRASSINOSTEROID INSENSITIVE 1-EMS-SUPPRESSOR 1 (BES1) and BRASSINAZOLE RESISTANT 1 (BZR1), belong to a small family with four BES1/BZR1 homologs (BEH1–4). To date, in contrast to the wealth of knowledge regarding BES1 and BZR1, little is known about BEH1–4. Here, we show that BEH2 was expressed preferentially in the roots and leaf margins including serrations, which was quite different from another member BEH4, and that BRs downregulated BEH2 through a module containing GSK3-like kinases and BES1/BZR1 TFs, among which BES1, rather than BZR1, contributed to this process. In addition, BEH2 consistently existed in the nucleus, suggesting that its subcellular localization is not under BR-dependent nuclear-cytoplasmic shuttling control. Furthermore, gene ontology analysis on RNA-seq data indicated that BEH2 may be implicated in stress response and photosynthesis. These findings might assist in the future elucidation of the molecular mechanisms underlying BR signaling.

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拟南芥BES1/BZR1同源物2在油菜素内酯信号传导中的行为和可能的功能

油菜素内酯(BR)信号传导不敏感的1- em - suppressor 1 (BES1)和抗油菜素唑1 (BZR1)的两个关键转录因子(TFs)属于一个小家族，有4个BES1/BZR1同源物(BEH1-4)。迄今为止，与关于BES1和BZR1的丰富知识相比，人们对BEH1-4知之甚少。我们发现BEH2优先表达在根和叶缘，包括锯齿，这与另一个成员BEH4有很大的不同，并且BRs通过包含gsk3样激酶和BES1/BZR1 tf的模块下调BEH2，其中BES1而不是BZR1参与了这一过程。此外，BEH2在细胞核中持续存在，表明其亚细胞定位不受br依赖的核细胞质穿梭控制。此外，对RNA-seq数据的基因本体论分析表明，BEH2可能与胁迫响应和光合作用有关。这些发现可能有助于未来阐明BR信号传导的分子机制。

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来源期刊

Plant Signaling & Behavior Agricultural and Biological Sciences-Plant Science

CiteScore

6.00

自引率

3.40%

发文量

111

期刊介绍： Plant Signaling & Behavior, a multidisciplinary peer-reviewed journal published monthly online, publishes original research articles and reviews covering the latest aspects of signal perception and transduction, integrative plant physiology, and information acquisition and processing.