ANTIMICROBIAL AND ANTICANCER ACTIVITIES OF EXTRACTS FROM URGINEA MARITIMA FRUITS

M. Obeidat, Ahmad Sharab
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引用次数: 4

Abstract

Background: Increasing antibiotic resistance among human pathogenic microorganisms and the failure of conventional cancer therapies attracting great attention among scientists in the field of herbal medicine to develop natural antimicrobial and anticancer drugs. Thus, the antimicrobial and anticancer activities from fruits of the medicinal plant Urginea maritima (L.) Baker that unexplored previously were investigated in this study. Materials and Methods: Fruits of U. maritima plant were collected, dried, ground, and extracted by hot water, ethanol, methanol, and acetone. The fruit extracts were examined for their potential as antimicrobial and anticancer agents using the agar well diffusion method and MTT assay, respectively. The gene expression of some cancer-related gene markers was determined by RT-PCR. Results: All fruit extracts of U. maritima exhibited antibacterial activity against S. aureus and E. coli. Methanol and ethanol extracts exhibited anticandidal activity. Ethanol and acetone extracts displayed non-hemolytic activity and selective cytotoxicity against breast cancer MCF7 cells with IC50 values that considered as active treatments. Concerning DNA fragmentation and gene expression after treatment of MCF7 cells with the most promising acetone extract, induction of apoptosis was proposed. The expression of cancer-related gene TNF after 6 hours, tumor suppressor genes (p53 and BRCA1), and immune response genes (IL-2 and IL-6) was induced. The expression of anti-apoptotic gene Bcl2 in treated MCF7 cells was reduced. Conclusion: Fruit extracts of U. maritima exhibited antimicrobial and anticancer activities. This result may lead to the use of these extracts for treatment of some infectious diseases and certain types of cancer.
海棠果实提取物的抑菌和抗癌活性研究
背景:人类病原微生物对抗生素耐药性的增加和传统癌症治疗的失败引起了草药领域科学家对开发天然抗菌和抗癌药物的高度关注。因此,药用植物海棠(L.)果实的抑菌和抗癌活性这项研究调查了以前未被探索过的贝克。材料与方法:采集海参果实,干燥、研磨,用热水、乙醇、甲醇和丙酮提取。分别采用琼脂孔扩散法和MTT法检测果实提取物的抑菌和抗癌作用。RT-PCR检测肿瘤相关基因标记的基因表达。结果:海参果实提取物对金黄色葡萄球菌和大肠杆菌均有抑菌活性。甲醇和乙醇提取物均表现出抗药活性。乙醇和丙酮提取物对乳腺癌MCF7细胞具有非溶血活性和选择性细胞毒性,IC50值被认为是积极的治疗方法。最具潜力的丙酮提取物对MCF7细胞的DNA断裂和基因表达有诱导凋亡的作用。6小时后,诱导肿瘤相关基因TNF、肿瘤抑制基因p53、BRCA1、免疫应答基因IL-2、IL-6的表达。抗凋亡基因Bcl2在MCF7细胞中表达降低。结论:海参果实提取物具有抗菌和抗癌活性。这一结果可能导致这些提取物用于治疗某些传染病和某些类型的癌症。
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来源期刊
自引率
0.00%
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0
审稿时长
6-12 weeks
期刊介绍: The “African Journal of Traditional, Complementary and Alternative Medicines (AJTCAM)” is a peer-reviewed, multidisciplinary, international, scientific Open Access Journal that provides publication of articles on phytomedicines, ethnomedicines and veterinary ethnomedicines. The journal is published by a Non-Governmental Organization (NGO) known as “African Traditional Herbal Medicine Supporters Initiative (ATHMSI)”. The Journal welcomes submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published approximately two-to-three months after acceptance
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