Cytotoxic and antioxidant activities of marine sponge diversity at Pecaron Bay Pasir Putih Situbondo East Java, Indonesia

Abstract

Objective

A study on the cytotoxic and antioxidant activities of Sponges collected from Pecaron Bay Pasir Putih Situbondo was conducted. This study aimed at screening the hydro-ethanolic extracts of 7 sponge species: Callyspongia sp, Acanthella sp and Xestospongia sp colleted at the Pecaron Situbondo, East Java, Indonesia for antiproliferative and antioxidant activities.

Methods

After collection, the species were immediately immersed in ethanol and stored at low temperatures (−20 °C) until use. Cytotoxic assay was conducted using MTT methods for some cancerous cells, including T47D, Casky and HT-29 meanwhile antioxidant assay was conducted using DPPH method.

Results

the extracts from species A. suberitoides has the highest toxicity compare to another species which valued level on tumor cell lines (HT-29, T47D and Casky). Antioxidant assay using DPPH method found that only Aaptos suberitoides that had been identified to show strong activity due to IC₅₀ value of <30 μg/mL; meanwhile Fascaplysinopsis reticulata, Acanthella sp, Petrosia contignata and Xestospongia exigua showed moderate antioxidant activity with a IC₅₀ < 100 μg/mL. Xestospongia sp, Callyspongia sp showed a value of IC₅₀ > 100 μg/mL.

Conclusion

The study showed that Xestospongia sp, Fascaplysinopsis reticulata, Callyspongia sp, Petrosia contignata, Aaptos suberitoides were highly potential to develop for isolation of bioactive compounds as anticancer and antioxidant agents.