Two typical acyl-CoA-binding proteins (ACBPs) are required for the asexual development and virulence of Phytophthora sojae.

IF 4.6 1区社会学 Q1 AREA STUDIES

Journal of Chinese Political Science Pub Date : 2022-07-01 Epub Date: 2022-05-02 DOI:10.1016/j.fgb.2022.103695

Yong Pei, Jierui Si, Natasha Navet, Peiyun Ji, Xiong Zhang, Huijun Qiao, Ruofei Xu, Ying Zhai, Jianqiang Miao, Brett M Tyler, Daolong Dou

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引用次数: 1

Abstract

Being found in all eukaryotes investigated, acyl-CoA-binding proteins (ACBPs) participate in lipid metabolism via specifically binding acyl-CoA esters with high affinity. The structures and functions of ACBP family proteins have been extensively described in yeasts, fungi, plants and mammals, but not oomycetes. In the present study, seven ACBP genes named PsACBP1-7 were identified from the genome of Phytophthora sojae, an oomycete pathogen of soybean. CRISPR-Cas9 knockout mutants targeting PsACBP1 and PsACBP2 were created for phenotypic assays. PsACBP1 knockout led to defects in sporangia production and virulence. PsACBP2 knockout mutants exhibited impaired vegetative growth, zoospore production, cyst germination and virulence. Moreover, Nile red staining of PsACBP2 knockout and over-expression lines showed that PsACBP2 is involved in the formation of lipid bodies in P. sojae. Our results demonstrate that two ACBP genes are differently required for growth and development, and both are essential for virulence in P. sojae.

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两种典型的酰基-CoA 结合蛋白（ACBPs）是索氏疫霉无性发育和毒力所必需的。

酰基-CoA 结合蛋白（ACBPs）以高亲和力特异性结合酰基-CoA 酯，参与脂质代谢。ACBP 家族蛋白的结构和功能已在酵母、真菌、植物和哺乳动物中得到广泛描述，但在卵菌中尚未发现。本研究从大豆的一种卵菌病原体 Phytophthora sojae 的基因组中鉴定出 7 个 ACBP 基因，命名为 PsACBP1-7。针对 PsACBP1 和 PsACBP2 创建了 CRISPR-Cas9 基因敲除突变体，用于表型检测。PsACBP1 基因敲除导致孢子囊产生和毒力缺陷。PsACBP2 基因敲除突变体的无性生殖、子囊孢子产生、子囊萌发和毒力均受到损害。此外，对 PsACBP2 基因敲除株和过表达株进行尼罗河红染色表明，PsACBP2 参与了 P. sojae 体内脂质体的形成。我们的研究结果表明，两种 ACBP 基因在生长和发育过程中的需要量不同，而且都对 P. sojae 的毒力至关重要。

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来源期刊

Journal of Chinese Political Science Multiple-

CiteScore

6.80

自引率

27.30%

发文量

期刊介绍： Journal of Chinese Political Science (JCPS) is a refereed academic journal that publishes theoretical, policy, and empirical research articles on Chinese politics across the whole spectrum of political science, with emphasis on Chinese domestic politics and foreign policy in comparative perspectives. However, JCPS also welcomes manuscripts on different aspects of contemporary China when these relate closely to Chinese politics, political economy, political culture, reform and opening, development, the military, law and legal system, foreign relations, and other important issues of political significance.