Biochemistry Method: Simultaneous determination of formaldehyde and methyl tert-buthyl ether in environmental and human biological matrices using static headspace gas chromatography mass spectrometry

Abstract

The present study describes a method based on static headspace- extraction (HS) followed by gas chromatography/ mass spectrometry (GC/MS) for the qualitative and quantitative analysis of Methyl tert-buthyl ether (MTBE) and formaldehyde (HCHO) in human blood and water samples. Cytochrome P4502A6 has important role for converting of MTBE to tert-butyl alcohol (TBA) and HCHO. To enhance the extraction capability of the HS, extraction parameters such as extraction temperature, extraction time, the ratio of headspace volume to sample volume and sodium chloride concentration have been optimized.