Investigation on Cell Surface Markers of Dental Pulp Stem Cell Isolated from Impacted Third Molar Based on International Society for Cellular Therapy Proposed Mesenchymal Stem Cell Markers

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2019-03-01 DOI:10.21705/MCBS.V3I1.34

F. Sandra, J. Sudiono, Yohanna Feter, Nadhia Sari Afiana, J. Chandra, Kharima Abdullah, Jasmine Shafira, A. Chouw

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引用次数: 1

Abstract

Background: Recently we have isolated and cultured dental pulp stem cell (DPSC) derived from impacted third molar (DPSC-M3). The DPSC-M3 was suggested as mesenchymal stem cell, however the cell surface markers were not completely clarified. Therefore current study was conducted to investigate the markers.Materials and Methods: Passage 5 DPSC-M3 was cultured, labeled and examined with flow cytometer. All markers were investigated according to the proposed cell surface marker panel for the minimal identification of human mesenchymal stem cell (MSC) by International Society for Cellular Therapy (ISCT). The positive markers were cluster of differentiation (CD)90, CD73, CD105, while the negative markers were CD34, CD45, CD11b, CD19, and Human Leukocyte Antigen (HLA)-DR. Results: Results showed that the size and granularity of DPSC-M3 were ranged from 75 to 230 and 27 to 203, respectively. The cell surface antigens examination showed that CD90, CD105 and CD73 were highly expressed (>95%), meanwhile expressions of CD45, CD34, CD11b, CD19 and HLA-DR were <2%.Conclusion: Since the all markers expression were in accordance to the proposed cell surface marker panel for the minimal identification of human MSC by ISCT, DPSC-M3 could be suggested as an MSC.Keywords: dental pulp, stem cell, dental pulp stem cell, ISCT, flow cytometry

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基于国际细胞治疗学会提出的间充质干细胞标记

背景:最近我们分离并培养了来自埋伏第三磨牙(DPSC- m3)的牙髓干细胞(DPSC)。DPSC-M3被认为是间充质干细胞，但细胞表面标记物尚未完全明确。因此，本研究开展了标记物的研究。材料与方法:培养第5代DPSC-M3，标记，流式细胞仪检测。根据国际细胞治疗学会(ISCT)提出的用于人类间充质干细胞(MSC)最小鉴定的细胞表面标记面板，对所有标记进行了研究。阳性标记物为cd90、CD73、CD105，阴性标记物为CD34、CD45、CD11b、CD19和人白细胞抗原(HLA)-DR。结果:结果显示DPSC-M3的粒径范围为75 ~ 230，粒度范围为27 ~ 203。细胞表面抗原检测显示CD90、CD105和CD73高表达(约95%)，而CD45、CD34、CD11b、CD19和HLA-DR表达均<2%。结论:由于所有标记的表达符合所提出的细胞表面标记面板，通过ISCT最低限度地鉴定人MSC，因此可以建议DPSC-M3为MSC。关键词:牙髓，干细胞，牙髓干细胞，ISCT，流式细胞术

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MCBS Molecular and Cellular Biomedical Sciences

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20 weeks