Simultaneous mutations in SMN1 and SUMM2 fully suppress the dwarf and autoimmune phenotypes of Arabidopsis mpk4 mutant

IF 2.8 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Signaling & Behavior Pub Date : 2022-03-30 DOI:10.1080/15592324.2022.2046412

Momoko Takagi, Suzuna Nagai, H. Kaminaka, K. Akimitsu, K. Shirasu, K. Ichimura

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引用次数: 3

Abstract

ABSTRACT Disruption of the Arabidopsis mitogen-activated protein kinase pathway, MEKK1–MKK1/MKK2–MPK4 (hereafter designated as MEKK1 pathway), leads to the activation of distinct NLRs (nucleotide-binding and leucine-rich repeat receptors), TNL (TIR-type NLR) SMN1, and CNL (CC-type NLR) SUMM2, resulting in dwarf and autoimmune phenotypes. Unlike mekk1 and mkk1mkk2 mutants, the dwarf and autoimmune phenotypes of mpk4 are only partially suppressed by the summ2 mutation, suggesting a significant contribution of SMN1 to the mpk4 phenotypes. However, full suppression of mpk4 by the smn1summ2 double mutation remains to be elucidated. To address this key question, we generated a mpk4smn1summ2 triple mutant and analyzed the dwarf and constitutive cell death phenotypes. The mpk4smn1summ2 triple mutant showed restoration of plant size with no detectable cell death, indicating full suppression of the dwarf and autoimmune phenotypes. These results suggest that SMN1 and SUMM2 constitute a robust surveillance system for the MEKK1 pathway against pathogen infection.

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SMN1和SUMM2的同时突变完全抑制拟南芥mpk4突变体的矮化和自身免疫表型

拟南芥丝裂原激活的蛋白激酶途径MEKK1 - mkk1 / MKK2-MPK4(以下称为MEKK1途径)的破坏，导致不同的NLRs(核苷酸结合和富含亮氨酸的重复受体)、TNL (tir -型NLR) SMN1和CNL (cc -型NLR) SUMM2的激活，导致矮化和自身免疫表型。与mekk1和mkk1mkk2突变体不同，summ2突变体仅部分抑制mpk4的侏儒和自身免疫表型，这表明SMN1对mpk4表型有重要贡献。然而，smn1summ2双突变对mpk4的完全抑制仍有待阐明。为了解决这个关键问题，我们生成了一个mpk4smn1summ2三重突变体，并分析了矮化和组成型细胞死亡表型。mpk4smn1summ2三突变体显示植株大小恢复，未检测到细胞死亡，表明矮化和自身免疫表型完全抑制。这些结果表明SMN1和SUMM2构成了MEKK1途径对抗病原体感染的强大监测系统。

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来源期刊

Plant Signaling & Behavior Agricultural and Biological Sciences-Plant Science

CiteScore

6.00

自引率

3.40%

发文量

111

期刊介绍： Plant Signaling & Behavior, a multidisciplinary peer-reviewed journal published monthly online, publishes original research articles and reviews covering the latest aspects of signal perception and transduction, integrative plant physiology, and information acquisition and processing.