Chlorpyrifos Mediated Amendment in Protein Profiling of Bacillus spp.

Abstract

Chlorpyrifos is a popularly used organophosphate and has immense agricultural applications. It is one of the major causes of soil contamination due to its higher adsorption coefficient, hydrophobicity and relatively longer persistence. Conducted study was attempted to monitor responses of Bacillus megaterium, isolated from the paddy growing agricultural field towards different doses of chlorpyrifos. The results highlighted the tolerance of this bacterium to higher concentration (800 mg L-1) of chlorpyrifos. However, protein is an important macromolecule in any living cells and is representative of all the important functions going inside the cell. Thus, quantification and profiling of protein of this bacterium in response to different doses of chlorpyrifos would probably decipher expression, if any, of stress responsive gene(s). The overall findings revealed that Bacillus megaterium expressed a few enzymes/ or proteins in order to show adaptation towards surrounding environment. It can be an efficient degrader of chlorpyrifos, hence, could be exploited for remediation of chlorpyrifos contaminated sites.