Lipid and Wall Amino Acid Composition in the Classification of Rothia dentocariosa

Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious... Pub Date : 1984-08-01 DOI:10.1016/S0174-3031(84)80054-2

T.M. Embley , M. Goodfellow , D.E. Minnikin , A.G. O'Donnell

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引用次数: 17

Abstract

Seven strains of Rothia dentocariosa were degraded by acid methanolysis and the non-hydroxylated fatty acid methyl esters released were examined by thin-layer and gas chromatography. The fatty acid profiles were composed of iso-, anteiso- and straight chain saturated fatty acids with 12-methyltetradecanoic (anteiso-C₁₅), 14-methylpentadecanoic (iso-C₁₆), 14-methylhexadecanoic (anteiso-C₁₇) and hexadecanoic acid (C₁₆) as major components. A small scale integrated procedure was used for the sequential extraction of isoprenoid quinones and polar lipids. The latter were examined by two-dimensional thin-layer chromatography and all of the test strains contained diphosphatidylglycerol, phosphatidylglycerol and two uncharacterised glycolipids. In all cases the major isoprenoid quinones were unsaturated menaquinones with seven isoprene units. Analyses of the cell wall amino acid composition using gas chromatography showed that the strains contained 2.5 to 5 moles of alanine and 1 mole each of glutamic acid and lysine. The chemical data support the integrity of Rothia dentocariosa and can be used to separate it from all other actinomycetes especially those which contain lysine in the wall peptidoglycan.

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牙齿罗汉属植物脂质和壁氨基酸组成的分类

采用酸甲醇分解法对7株牙齿罗汉果进行降解，并采用薄层色谱和气相色谱法检测其释放的非羟基脂肪酸甲酯。脂肪酸谱由异链饱和脂肪酸、异链饱和脂肪酸和直链饱和脂肪酸组成，主要成分为12-甲基十四烷酸(anteiso- c15)、14-甲基五烷酸(isoc16)、14-甲基十六烷酸(anteiso- c17)和十六烷酸(C16)。采用小型综合工艺，对类异戊二烯醌类和极性脂类进行了序贯提取。后者采用二维薄层色谱法检测，所有试验菌株均含有二磷脂酰甘油、磷脂酰甘油和两种未表征的糖脂。在所有情况下，主要的类异戊二烯醌是具有7个异戊二烯单位的不饱和甲基萘醌。用气相色谱法分析细胞壁氨基酸组成表明，菌株含有2.5 ~ 5 mol的丙氨酸，1 mol的谷氨酸和1 mol的赖氨酸。化学数据支持牙齿罗氏菌的完整性，并可用于将其与所有其他放线菌分离，特别是那些在壁肽聚糖中含有赖氨酸的放线菌。

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Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...

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