Micropropagation of Ficus carica L. through Tissue Culture System

Patah, H. Idris, Radzuan, M. Lassim
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引用次数: 1

Abstract

Ficus spp. is one of Moraceae family which natively to Westerns Asia and distributed worldwide and economically important. The richness of nutrients and latex cells make this plant possessed many pharmacological effects which is importantly to medicinal and drugs industries. The demand for this plant is very high despite the production of fig is very inconsistent from year to year. So, this study have determined the highest shoot regeneration recorded when the stem explant was cultured in MS medium supplemented with 1.0 mg/L of BAP + 0.0 g/L NAA with 2.57±0.997. For callus induction, the highest fresh weight in stem explant was 2.28±0.239 obtained when cultured in MS medium supplemented with 0.5 mg/L BAP + 1.0 mg/L NAA, shoot explant was 1.0 mg/L BAP + 0.5 mg/L NAA with fresh weight 2.39±0.134 g, petiole explant was 1.42±0.230 g in MS medium supplemented with 0.5 mg/L BAP + 1.0 mg/L NAA and root explant was was 1.43±0.148 g when explant was cultured on 1.0 mg/L BAP + 1.0 mg/L NAA.
榕树组织培养系统的微繁研究
榕属植物是桑科植物之一,原产于西亚,分布在世界各地,具有重要的经济意义。其丰富的营养成分和乳胶细胞使其具有许多药理作用,对医药工业具有重要意义。尽管无花果的产量每年都不稳定,但对这种植物的需求非常高。因此,本研究确定了茎外植体在添加1.0 mg/L BAP + 0.0 g/L NAA(2.57±0.997)的MS培养基中培养的再生率最高。对于愈伤组织的诱导,茎外植体在添加0.5 mg/L BAP + 1.0 mg/L NAA的MS培养基中培养时鲜重最高,为2.28±0.239,茎外植体为1.0 mg/L BAP + 0.5 mg/L NAA,鲜重2.39±0.134 g,叶柄外植体在添加0.5 mg/L BAP + 1.0 mg/L NAA的MS培养基中培养时鲜重1.42±0.230 g,根外植体在添加1.0 mg/L BAP + 1.0 mg/L NAA的MS培养基中培养时鲜重1.43±0.148 g。
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