Poly(3-hydroxybutyrate) Production from Natural Gas by a Methanotroph Native Bacterium in a Bubble Column Bioreactor

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Chemical and Biochemical Engineering Quarterly Pub Date : 2019-04-10 DOI:10.15255/CABEQ.2017.1263

Kianoush Kosravi-Darani, F. Yazdian, Fatemeh Babapour, A. Amirsadeghi

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引用次数: 12

Abstract

Reducing the total cost of poly(3-hydroxybutyrate) (PHB) production as an attractive substitute for conventional petrochemical plastics still remains an unsolved problem. The aim of this research was the screening of PHB-producing microorganisms and selection of the best suitable medium for microbial growth and PHB production from methane. A new isolated methanotroph for PHB production from natural gas was studied in different media. After selection of the suitable medium, the effect of five process variables (content of nitrogen source, disodium hydrogen phosphate, methane to air ratio, seed age, and pH) on PHB production was investigated in a bubble column bioreactor. Also, hydrodynamic and mass transfer factors (flow regime, mixing time, gas hold up, and kLa) were considered. At optimum operating conditions and engineering parameters in a bubble column, PHB content in the dried biomass reached 25 % w/w. The results showed that pH is the most important variable in the selected conditions.

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气泡塔生物反应器中甲烷化原生细菌从天然气中生产聚(3-羟基丁酸酯

降低聚(3-羟基丁酸酯)(PHB)生产的总成本，作为一种有吸引力的常规石化塑料替代品，仍然是一个未解决的问题。本研究的目的是筛选产生PHB的微生物，选择微生物生长和甲烷生产PHB的最佳培养基。研究了一种分离的新型甲烷化菌在不同介质下从天然气中生产PHB。在选择合适的培养基后，在气泡塔生物反应器中考察了氮源含量、磷酸氢二钠含量、甲烷空气比、种子年龄和pH值等5个工艺变量对PHB生产的影响。此外，还考虑了流体动力学和传质因素(流动状态、混合时间、气含率和kLa)。在气泡塔的最佳操作条件和工程参数下，干燥生物质中PHB含量达到25% w/w。结果表明，pH是选择条件中最重要的变量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical and Biochemical Engineering Quarterly 工程技术-工程：化工

CiteScore

2.70

自引率

6.70%

发文量

审稿时长

>12 weeks

期刊介绍： The journal provides an international forum for presentation of original papers, reviews and discussions on the latest developments in chemical and biochemical engineering. The scope of the journal is wide and no limitation except relevance to chemical and biochemical engineering is required. The criteria for the acceptance of papers are originality, quality of work and clarity of style. All papers are subject to reviewing by at least two international experts (blind peer review). The language of the journal is English. Final versions of the manuscripts are subject to metric (SI units and IUPAC recommendations) and English language reviewing. Editor and Editorial board make the final decision about acceptance of a manuscript. Page charges are excluded.