Effects of Experemental Cerebral Ishemia on Metabolic Characteristics of Parietal Cortex Neurons

Abstract

The aim of the paper was to estimate the effect of experimental cerebral ischemia on metabolic characteristics of parietal cortex neurons. The experiments were carried out on 12 laboratory male rats. Cerebral ischemia was modeled with bilateral joint carotid arteries with durations of 60 minutes under intravenous thiopental anesthesia (40-50 mg / kg). The rats were decapitated and the samples of the brain parietal cortex were prepared for histological and histochemical examination in combination with morphometry to examine the 5 th layer inner pyramidal neurons. It was increased the number of pathological forms and shrinkage of the 5 th layer inner pyramidal neurons after cerebral ischemia in rats. The histochemical examination was revealed the inhibition of NADH-, glucose-6-phosphate dehydrogenase and succinate dehydrogenases as well as activation of lactate dehydrogenase and acid phosphatase. Cerebral ischemia induces deep histological and histochemical changes in the parietal cortex neurons in rats, including shrinkage of parietal cortex neurons and disturbances of their energy metabolism.