{"title":"Induction of phenylalanine ammonia lyase (PAL) in gamma irradiated potatoes","authors":"M.B. Pendharkar, P.M. Nair","doi":"10.1016/S0033-7560(75)80007-X","DOIUrl":null,"url":null,"abstract":"<div><p>The exposure of potatoes to gamma rays (10 krad) caused on induction of phenylalanine ammonia lyase (PAL) in the tubers. The induced activity was localized essentially in cortex tissue, mainly concentrated around the bud region. Whereas in parenchymatous tissue no induction was observed. The enhancement in PAL activity in buds was observed to be dependent on dose of irradiation and the duration of storage thereafter. The tubers exposed to a 10 krad dose showed maximal activation on storage at ambient conditions with 20 fold activity observed within three hours after irradiation. On further storage the activity declined and reached a constant level within a period of one week.</p><p>The increase in PAL activity was partly due to activation of the inactive form of the enzyme (30–40%) and partly due to <em>de nova</em> synthesis of the protein (60–70%).</p></div>","PeriodicalId":20794,"journal":{"name":"Radiation Botany","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1975-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0033-7560(75)80007-X","citationCount":"30","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Radiation Botany","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S003375607580007X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 30
Abstract
The exposure of potatoes to gamma rays (10 krad) caused on induction of phenylalanine ammonia lyase (PAL) in the tubers. The induced activity was localized essentially in cortex tissue, mainly concentrated around the bud region. Whereas in parenchymatous tissue no induction was observed. The enhancement in PAL activity in buds was observed to be dependent on dose of irradiation and the duration of storage thereafter. The tubers exposed to a 10 krad dose showed maximal activation on storage at ambient conditions with 20 fold activity observed within three hours after irradiation. On further storage the activity declined and reached a constant level within a period of one week.
The increase in PAL activity was partly due to activation of the inactive form of the enzyme (30–40%) and partly due to de nova synthesis of the protein (60–70%).
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