Study of parameters of lipid peroxidation and antioxidant protection in rats treated with cadmium nitrate with biocorrection by infusion of Beta vulgaris seeds

Abstract

Recently, interest in natural antioxidants and their use in the food industry has increased. In addition to ensuring maximum safety of the produced product, they also serve as an antioxidant shield for the entire living organism. Plant extracts contain flavonoid compounds that have an antioxidant effect. In this regard, the purpose of the article is to study the antioxidant properties of infusion of Beta vulgaris’s seeds, to establish the relationship between acute and subacute inoculation of rats with cadmium nitrate and oxidative stress. For this experimental study, we used 50 male rats. The animals were exposed to Cd nitrate, with an initial weight of 180 ± 30 g. The first groups of rats were injected with cadmium nitrate at a dose of 0.1 g/L inter peritoneal, the second groups received a 0.01% solution of cadmium nitrate 1 ml 5 days a week, orally for 10 and 24 days. The state of oxidative metabolism in the blood was investigated. To assess the state of oxidative metabolism in the blood, the primary, secondary and end products of lipid peroxidation were determined, such as diene conjugates (DC), cetodiene (CD), malondialdehyde (MDA), Schiff bases (SHB), and the activity of glutathione peroxidase (GP), catalase (CAT), and adenosine deaminase (ADA) enzymes were evaluated by the parameters of antioxidant protection. The mean ± SEM values were calculated for each group to determine the significance of the intergroup difference. Each parameter was analyzed separately using the one-way analysis of variance (ANOVA) test. To determine the difference between groups, Student’s “t”-test was used. Studies have shown that with the use of cadmium in the blood of experimental animals, there is a violation of catabolits of lipoperoxidation, depending on the length of the days of poisoning, but a significant decrease in the biocorrection of Beta vulgaris’s seeds. Because of in this study, we found that the activity levels of antioxidant defense enzymes were significantly increased in the blood of experimental animals, which biocorrected with beetroot seed infusion. In conclusion, our study allows us to state that the use infusion of Beta vulgaris’s seeds in subacute and acute experiments with poisoning of experimental rats with cadmium nitrate as an antioxidant protective effect.