Oxidative Stress Induced DNA Damage and Reproductive Toxicity in Male Albino Mice Orally Exposed to Sorbitol

Annals of Science and Technology Pub Date : 2019-12-01 DOI:10.2478/ast-2019-0010

O. Alabi, Lanre R. Oladimeji, A. A. Sorungbe, Yetunde M. Adeoluwa

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引用次数: 1

Abstract

Abstract In this study, the potential DNA damage and reproductive toxicity of sorbitol was investigated using bone marrow micronucleus (MN), sperm morphology, and sperm count in mice. Five doses of 90, 45, 20, 10 and 1 mg/kg/day, defined by allometry, and approximately corresponding to 1.5g, 750mg, 330mg, 165mg and 16mg of sorbitol daily consumption by a 70kg human, respectively, were used. MN analysis showed a dose-dependent induction of micronucleated polychromatic erythrocytes and other nuclear abnormalities across the treatment groups. Assessment of sperm shape showed a significant (p < 0.05) increase in sperm abnormalities with significant (p < 0.05) decrease in mean sperm count in treated groups. The result of the oxidative stress biomarkers showed induction of significant (p < 0.05) increase in liver catalase, MDA and serum ALT and AST activities with concomitant decrease in SOD activities in exposed mice. A significant increase in weight of exposed mice were recorded when compared with the negative control. The results of this study showed the genotoxicity and reproductive effects of sorbitol.

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氧化应激诱导的雄性白化小鼠口服山梨醇的DNA损伤和生殖毒性

摘要本研究通过小鼠骨髓微核(MN)、精子形态和精子数量研究山梨糖醇的潜在DNA损伤和生殖毒性。根据异速测量法确定的90、45、20、10和1 mg/kg/天5个剂量，分别约对应于70kg人每天1.5g、750mg、330mg、165mg和16mg的山梨醇摄入量。MN分析显示，剂量依赖性诱导微核多染红细胞和其他核异常的治疗组。精子形状评估显示，治疗组精子异常显著(p < 0.05)增加，平均精子数量显著(p < 0.05)减少。氧化应激生物标志物结果显示，氧化应激诱导小鼠肝脏过氧化氢酶、丙二醛和血清ALT、AST活性显著(p < 0.05)升高，SOD活性降低。与阴性对照相比，暴露小鼠的体重显著增加。本研究结果显示山梨醇的遗传毒性和生殖效应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Annals of Science and Technology

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