An Investigation into the Effect of the Nitric Oxide Synthase Antagonist L-NAME and Plant Extracts on the Irritability and Barrier Function of the Skin

A. Ormerod, P. Woo, J. Islam, J. Moran, M. Cals-Grierson
{"title":"An Investigation into the Effect of the Nitric Oxide Synthase Antagonist L-NAME and Plant Extracts on the Irritability and Barrier Function of the Skin","authors":"A. Ormerod, P. Woo, J. Islam, J. Moran, M. Cals-Grierson","doi":"10.1159/000081566","DOIUrl":null,"url":null,"abstract":"Background: Nitric oxide (NO) synthesis is upregulated in dermatitis and may be involved in the modulation of keratinocyte proliferation and formation of the cornified envelope. Objective: To examine the possible effects of modulators of NO production on erythema and transepidermal water loss (TEWL) induced by sodium lauryl sulphate (SLS). Methods: Ten volunteers were patch tested with water and SLS 0.2, 0.5 and 1%. The outcomes of TEWL and erythema were assessed at 1, 6, 25 and 49 h. The effects of the NO inhibitors, L-NAME, extracts of Olea europa and Ginkgo biloba (as 1% gel solutions) and of the NO donor, glyceryl trinitrate (as 2% ointment) were evaluated. Results: L-NAME and the plant extracts reduced TEWL. The TEWL induced by 0.5% SLS was significantly diminished by L-NAME at 1 and 6 h and by ginkgo extract at 6 and 49 h. Erythema was too mild for reflectance measurements to show significant inhibition, but erythema scores tended to be lower at 6 h the application of NO inhibitors. Conclusion: NO inhibitors may protect skin barrier function from the adverse effects of chemical irritants.","PeriodicalId":12086,"journal":{"name":"Exogenous Dermatology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Exogenous Dermatology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1159/000081566","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

Abstract

Background: Nitric oxide (NO) synthesis is upregulated in dermatitis and may be involved in the modulation of keratinocyte proliferation and formation of the cornified envelope. Objective: To examine the possible effects of modulators of NO production on erythema and transepidermal water loss (TEWL) induced by sodium lauryl sulphate (SLS). Methods: Ten volunteers were patch tested with water and SLS 0.2, 0.5 and 1%. The outcomes of TEWL and erythema were assessed at 1, 6, 25 and 49 h. The effects of the NO inhibitors, L-NAME, extracts of Olea europa and Ginkgo biloba (as 1% gel solutions) and of the NO donor, glyceryl trinitrate (as 2% ointment) were evaluated. Results: L-NAME and the plant extracts reduced TEWL. The TEWL induced by 0.5% SLS was significantly diminished by L-NAME at 1 and 6 h and by ginkgo extract at 6 and 49 h. Erythema was too mild for reflectance measurements to show significant inhibition, but erythema scores tended to be lower at 6 h the application of NO inhibitors. Conclusion: NO inhibitors may protect skin barrier function from the adverse effects of chemical irritants.
一氧化氮合酶拮抗剂L-NAME与植物提取物对皮肤过敏性和屏障功能影响的研究
背景:一氧化氮(NO)合成在皮炎中上调,可能参与角化细胞增殖和角化包膜形成的调节。目的:探讨一氧化氮调节剂对十二烷基硫酸钠(SLS)所致红斑和经皮失水(TEWL)的影响。方法:10名志愿者分别用水、0.2、0.5、1%的SLS贴片试验。在第1、6、25和49小时评估TEWL和红斑的结果。评估NO抑制剂L-NAME、油橄榄和银杏提取物(作为1%凝胶溶液)和NO供体三硝酸甘油(作为2%软膏)的效果。结果:L-NAME和植物提取物均能降低TEWL。0.5% SLS诱导的TEWL在1和6 h时被L-NAME显著降低,在6和49 h时被银杏提取物显著降低。红斑过于轻微,无法进行反射测量,但在使用NO抑制剂6 h时,红斑评分往往较低。结论:一氧化氮抑制剂可保护皮肤屏障功能免受化学刺激物的不良反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信