Reproducibility of Somatic Embryogenesis Upon Resampling of Oil Palm Ortets

The Planter Pub Date : 2023-08-29 DOI:10.56333/tp.2023.025
C. Lim, Foo-Hin Wong, S. Ooi
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Abstract

Somatic embryogenesis remains to be a key bottleneck in oil palm clonal propagation and resampling of embryogenic ortets may be one approach to alleviate this problem. Resampling, is currently practised by oil palm tissue culture laboratories to increase the production of clones from elite ortets. However, reproducibility of the embryogenesis outcome from resampling exercises has not been examined in detail. Therefore, embryogenesis reproducibility upon resampling was evaluated using the concordance correlation coefficient analysis. Sampling and resampling were conducted when ortets were seven to 19 years old. Resampling interval periods of three to eight years showed moderate concordance in embryogenesis reproducibility. An alternative analysis approach through categorising the ortets based on their embryogenesis outcomes into zero, low, medium and high embryogenesis categories indicated that 54.4 per cent of ortets reproduced their embryogenesis outcome while 35.6 per cent showed a shift by one embryogenesis category upon resampling. A resampling interval of 10 years was associated with a decline in embryogenesis outcomes. Observations from this study may help tissue culture laboratories in refining the selection of ortets for clonal propagation with the possibility of improving success rates in resampling exercises and thereby reducing operational costs. Keywords: Ortet, concordance, non-embryogenic, sampling interval.
油棕羊体细胞胚胎发生的再现性研究
体细胞胚胎发生一直是油棕无性系繁殖的瓶颈,对胚性小体进行重采样可能是解决这一问题的途径之一。重新取样是目前油棕组织培养实验室采用的方法,以增加优质油棕的克隆产量。然而,从重新取样练习胚胎发生结果的可重复性尚未被详细检查。因此,用一致性相关系数分析来评价重采样后胚胎发生的可重复性。在孩子7岁到19岁之间进行了抽样和再抽样。重新采样的间隔时间为3 ~ 8年,在胚胎发生再现性方面表现出适度的一致性。另一种分析方法是根据胚胎发生的结果将小鼠分为零胚胎发生、低胚胎发生、中胚胎发生和高胚胎发生类别,结果表明54.4%的小鼠再现了它们的胚胎发生结果,而35.6%的小鼠在重新采样时发生了一个胚胎发生类别的变化。10年的重新采样间隔与胚胎发生结果的下降有关。本研究的观察结果可能有助于组织培养实验室改进对无性系繁殖的选择,并有可能提高重采样练习的成功率,从而降低操作成本。关键词:Ortet,一致性,非胚性,采样间隔。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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