Green synthesis of silver nanoparticles from Endophytic fungus Aspergillus niger isolated from Simarouba glauca leaf and its Antibacterial and Antioxidant activity

B. Hemashekhar, P. ChandrappaC, M. Govindappa, N. Chandrasekhar, Nagaraju Ganganagappa, Y. L. Ramachandra
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引用次数: 19

Abstract

The field of nanotechnology is the most promising area of the research. In our present study we report the biological method of synthesis of silver nanoparticles by endophytic extracts isolated from the leaf of Simarouba glauca. The surface Plasmon resonance characteristic of silver nanoparticles was revealed by the UV-Vis spectrum at 400 nm. The crystalline nature of silver nanoparticle was confirmed by X ray diffraction studies. Spherical shaped and monodispersed nanoparticles were found in Scanning electron micrograph. The average size of silver nanoparticles was 41.9 nm as determined by dynamic light scattering. The peak in silver region confirming the presence of elemental silver was determined by Energy dispersive X-ray spectroscopy analysis. Antibacterial activity of silver nanoparticles utilized in this study was found to be more significant than standard Taxim antibiotic against multidrug resistant bacteria such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumonia. Endophytic based silver nanoaprticles were found to possess significant antioxidant activity.
黑曲霉绿色合成纳米银颗粒及其抑菌抗氧化活性研究
纳米技术是该领域最有前途的研究领域。在本研究中,我们报道了从青叶中分离的内生提取物合成纳米银的生物方法。利用紫外可见光谱在400 nm处揭示了银纳米粒子的表面等离子体共振特性。通过X射线衍射研究证实了纳米银的结晶性质。扫描电镜观察到球形、单分散的纳米颗粒。通过动态光散射测定,银纳米粒子的平均尺寸为41.9 nm。通过能量色散x射线光谱分析确定了银区的峰,证实了银元素的存在。研究发现,银纳米颗粒对多重耐药细菌如大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌和肺炎克雷伯菌的抗菌活性比标准的Taxim抗生素更显著。内生银纳米颗粒具有显著的抗氧化活性。
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