In vitro Production of Multiple Seedlings From Single Seeds of Mung Bean (Vigna radiata L. Wilczek)

Abstract

A new procedure for obtaining more than one seedling per seed in Mung bean (Vigna radiata) is developed. This involves excision and isolation of both cotyledons from imbibed seeds and culturing them individually on a basal medium. Cotyledons directly developed into complete plants with roots. The percentage of the cotyledonary plants that developed was inversely proportional to the imbibition period of the seed. Cotyledon derived plants were transplanted to soil and raised to maturity. Normal pod development with seeds was observed in the transplanted plants. Seeds obtained from such plants were grown again in the field and the progenies obtained were normal. The cotyledon derived plants were comparable to seed raised plants.