The Influence of Technical Factors on the NBT Test

Abstract

The results of NBT tests were found to vary depending on how the tests were performed. Heparin increased the proportion of NBT positive neutrophils and the lowest possible concentration should be used. Blood samples for the NBT test could be stored for 8 h at 4° C with only a minor effect on the test results. The incubation time was crucial and 25 min in a 37° C water bath was adopted as standard procedure.