Molecular Heterogeneity of Salmonella Gene Isolated from Chickens, Ducks, Pigeons, and Rabbits in Egypt

Abstract

A total 250 fecal samples were collected from chicken, duck, pigeon, and rabbits for detection of Salmonella species. Salmonella species were detected in 42 out of 250 samples 16.8% and the most predominant species were Salmonella Enteritis was detected in 11 out 42 (26.2%) of total isolates and Salmonella Typhimurum was detected in 9 out 42 (21.4% ) of total isolates. All isolates were examined for antibiotics sensitivity by using disc diffusion technique against 10 different antibiotics Ampicillin , Amoxicillin-clavulanic acid , ciprofloxacin , cefotaxime , ceftriaxone , doxycycline , Norfloxacin , streptomycin , tetracycline and Gentamicin), and found that highest percentage of resistance in all species was seen with amoxicillin with clavulanic acid and the lowest percentage of resistance was seen with gentamicin, ciprofloxacine and norfloxacine. The molecular identification of virulence-associated genes in Salmonella strains which isolated from different poultry species and rabbits (n=42)revealed the detection of invA, , mgtC, bcfC, sopBand ssaG genes in 100% of the examined isolates, while avrA gene found to be in 90.5%Analyzing of nucleotide sequence of sopB virulence gene of identities between the isolated Egyptian Salmonella strains and different Salmonella strains uploaded from gene bank from different places all over the world revealed a 98.6% to 100% homology.