Flow-injection immunoanalysis, a versatile and powerful tool for the automatic determination of environmental pollutants

Laboratory Robotics and Automation Pub Date : 1999-04-19 DOI:10.1002/(SICI)1098-2728(1999)11:2<83::AID-LRA3>3.0.CO;2-H

Zheng-Liang Zhi

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引用次数: 2

Abstract

Flow-injection immunoanalysis (FIIA) is a novel and automatic technique combining flow-injection approach with specific antibodies to complement the step of an immunoassay in a flow-through column format. This short review focuses on new FIIA contributions for environmental analysis problems with special emphasis on the pesticide residue determinations. Several major strategies in development of such technique with respect to the preparation of the antibody, immunoreactor support selection, novel coupling procedures for antibody and antigen immobilization, immunobinding conditions and elution and regeneration protocols, as well as assay formats and trace and detection schemes are taken into account. Some novel applications of the FIIA that appeared in more recent years as related to the field of pesticide residue analysis in environmental waters are outlined. © 1999 John Wiley & Sons, Inc. Lab Robotics and Automation 11: 83–89, 1999

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流动注射免疫分析，一个多功能和强大的工具，自动测定环境污染物

流动注射免疫分析(FIIA)是一种新颖的自动技术，将流动注射方法与特异性抗体相结合，以补充流动柱格式的免疫分析步骤。本文简要综述了FIIA在环境分析问题上的新贡献，特别强调了农药残留的测定。考虑到开发这种技术的几个主要策略，包括抗体的制备，免疫反应器支持选择，抗体和抗原固定的新型偶联程序，免疫结合条件和洗脱和再生方案，以及分析格式和痕量和检测方案。概述了近年来FIIA在环境水体农药残留分析领域的一些新应用。©1999 John Wiley &儿子,Inc。实验室机器人与自动化(11):83-89,1999

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Laboratory Robotics and Automation

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