The Role of the Cytoskeleton in the Pairing and Positioning of the Two Nuclei in the Apical Cell of the Dikaryon of the Basidiomycete Coprinus cinereus

Takashi Kamada, Katsue Hirai, Motohiro Fujii
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引用次数: 21

Abstract

Kamada, T., Hirai, K., and Fujii, M. 1993. The role of the cytoskeleton in the pairing and positioning of the two nuclei in the apical cell of the dikaryon of the basidiomycete Coprinus cinereus. Experimental Mycology 17, 338-344. We examined the effects of tubulin mutations, the anti-microtubule agent benomyl, and the anti-microfilament agents cytochalasin B and E on the pairing and positioning of the two nuclei in the dikaryotic apical cell of the basidiomycete Coprinus cinereus. In the parental wild-type dikaryon, the two nuclei maintain an average separation of 19 μm and an average distance of about 76 μm from the growing hyphal apex; there was a weak tendency that the apex to nucleus distance decreases as cell length decreases. Examination of eight dikaryons homozygous for either one α- or one of seven β-tubulin mutations revealed that all the tubulin mutations disturbed the pairing of the two nuclei without changing the positioning of the leading nucleus. The anti-microtubule agent benomyl disturbed the nuclear pairing without changing the positioning of the leading nucleus. Neither cytochalasin B nor E affected either the pairing or the positioning. These results demonstrate that microtubules participate in the pairing of the two nuclei in the apical cell of the dikaryon and provide evidence against a direct involvement of either microtubules or microfilaments in the mechanism of their positioning relative to the hyphal apex.

细胞骨架在担子菌双核顶细胞两个细胞核配对和定位中的作用
Kamada, T., Hirai, K.和Fujii, M. 1993。细胞骨架在担子菌双核顶细胞两个细胞核配对和定位中的作用。真菌学通报,17(3):338-344。我们研究了微管蛋白突变、抗微管剂苯甲酰基和抗微丝剂细胞松弛素B和E对担子菌双核尖细胞两个细胞核配对和定位的影响。在亲本野生型双核中,两个核与生长菌丝顶端的平均距离为19 μm,平均距离约为76 μm;随着细胞长度的减小,顶端到细胞核的距离有较弱的减小趋势。对8个二核纯合子α-或7个β-微管蛋白突变中的一个进行检测,发现所有的微管蛋白突变都干扰了两个核的配对,但没有改变前导核的位置。抗微管剂苯甲酰干扰核配对,但不改变前导核的位置。细胞松弛素B和E既不影响配对也不影响定位。这些结果表明,微管参与了二核体顶端细胞的两个细胞核配对,并提供了证据,证明微管或微丝直接参与了它们相对于菌丝顶端的定位机制。
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