A Comparative Assessment of Pigment Extraction from Avian Eggshells Using Two Analytical Protocols

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
J. Gómez, C. Ramo, Juan A. Canales, I. García, M. Castro, Alejandro Pérez-Hurtado, J. Amat
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Abstract

There are two main methods to extract pigments from avian eggshells. There are those using strong acids (e.g. sulphuric, hydrochloric or formic), that allow for a quantification of pigment concentrations but with which samples are destroyed. Alternatively, there are methods employing weaker acids (e.g. acetic), with which samples are not destroyed, although pigment concentrations may not be quantified and only their presence is detected. In some cases, there may be a need to quantify pigments in samples in which the pigments have already been detected. Here, we assess whether the quantity of pigments extracted from eggshells using a strong acid is affected by previous treatment of eggshells with a weak acid. For this, we used eggshells of Kentish Plovers Charadrius alexandrinus and domestic Japanese Quails Coturnix japonica, and aliquots of individual eggshells treated either with both acids (first applying a weak acid and then a strong acid) or only with the strong acid. Quantities of extracted protoporphyrin and biliverdin (the main eggshell pigments) were lower when using a strong acid after application of a weak acid. However, there were significant positive correlations between the quantities of pigments extracted using the two methods, suggesting that eggshell samples used to determine pigment presence may still be usable to quantify pigment concentrations using a strong acid.—Gomez, J., Ramo, C., Canales, J.A., Garcia, I.M., Castro, M., Perez-Hurtado, A. & Amat, J.A. (2020). A comparative assessment of pigment extraction from avian eggshells using two analytical protocols.
两种分析方法提取禽蛋壳色素的比较研究
从禽蛋壳中提取色素主要有两种方法。有些使用强酸(如硫酸、盐酸或甲酸),可以定量测定色素浓度,但会破坏样品。另外,也有使用较弱酸(如乙酸)的方法,样品不会被破坏,尽管色素浓度可能无法定量,仅检测其存在。在某些情况下,可能需要对已经检测到的样品中的色素进行定量。在这里,我们评估了使用强酸从蛋壳中提取的色素的数量是否受到先前用弱酸处理蛋壳的影响。为此,我们使用了肯特鸻(Charadrius alexandrinus)和国内日本鹌鹑(Coturnix japonica)的蛋壳,并用两种酸(先用弱酸,然后用强酸)或只用强酸处理过等量的单个蛋壳。原卟啉和胆绿素(主要的蛋壳色素)的提取量在弱酸处理后用强酸处理时较低。然而,使用这两种方法提取的色素数量之间存在显著的正相关关系,这表明用于确定色素存在的蛋壳样品可能仍然可用强酸来量化色素浓度。-Gomez, J., Ramo, C., Canales, J.A., Garcia, i.m., Castro, M., Perez-Hurtado, A.和Amat, J.A.(2020)。两种分析方法对禽蛋壳色素提取的比较评价。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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