Screening a novel high effective dioscorea zingiberensis degradation strain

Abstract

12 strains of cellulolytic microorganism were isolated from the AB reactor of dioscorea zingiberensis wastewater treatment. A bacterial strain was obtained through the screening experiments of decomposition of dioscorea zingiberensis, which had high degradability for dioscorea zingiberensis and was named D-1. The gene sequence obtained from the DNA extraction and polymerase chain reaction (PCR) amplification was compared with NCBI Genbank of U.S.A., and the strain was preliminary identified to be Clostridium cellulosolvens. The optimum growth conditions were pH 5.0, 30°C, 100 r/min.