In Vitro Micropropagation of Rose (Hybrid Rosa spp.) through Plant Tissue Culture Technique

Abstract

Roses are the most important cut flowers in the world. Tissue culture of rose has been improved since last twenty years, and exploited for various purposes from basic anatomical and physiological research to micropropagation from auxiliary buds, shoot tips, leaf explants, etc. Single bud nodal stem segments were surface sterilized and then cultured on MS medium supplemented with 30 g/l sugar, 6 g/l agar and different concentrations of BAP(1, 2, 3, 4 and 5mg/l). Among them, the most suitable concentration for shoot initiation and multiplication was 3mg/l BAP. And it also found that the best for shoot elongation occurred in MS medium supplemented with BAP 3 mg/l and GA3 1mg/l. The proliferated microshoots were transferred to root inducing media of half strength MS medium supplemented with 15 g/l sugar, 5 g/l agar and NAA (0.5, 1, 1.5, 2 mg/l). The best rooting was observed at NAA 1 mg/l concentration. The least response in root formation was found at NAA 0.5 mg/l supplementation on half strength MS media.