Preparation, Characterization and Antiproliferation Evaluation of Nanoliposome Loaded with Anthocyanins

Abstract

This study aimed to investigate the nanoliposomes composed of soy lecithin and cholesterol for encapsulation of anthocyanins (ACNs) by thin film ultrasonic dispersion method. The processing conditions of ACNs nanoliposomes (ANLs) were optimized, and the ANLs were characterized by average particle size, zeta (ζ)-potential and polydispersity index (PDI). Their storage stability under various pH, temperature, light conditions, and in vitro antiproliferative effect were analyzed. The optimal preparation parameters were suggested to be soy lecithin to cholesterol ratio of 80 mg to 19 mg (w/w, 2 mg of ACNs) by using ultrasonication at 120 W for 3.12 min. The produced ANLs had the encapsulation efficiency of 40.1 % with average particle size of 117 nm, PDI of 0.254 and ζ-potential of 8.56 mV. These ANLs presenting ≈ 130 nm of stable sizes at tested pH conditions. The ACNs showed better in vitro antiproliferative effect for two cancer cell lines than ANLs, while ANLs were more stable against temperature and light. The ACNs in nanoliposomes displayed higher retention efficiency than that of in solution under various storage conditions. This study provides a promising approach for stabilizing ACNs-containing food ingredients, which displays large potentiality in the productions of nutraceutical and functional foods.