Methods Introduction of Transgenic Animal

Abstract

At present, the most common used method for producing transgenic animal is known as DNA microinjection. It directly injects the exogenous gene into fertilized egg cells by using a syringe with micromanipulation instrument. With the exogenous gene integrated into DNA, a transgenic animal is produced. The founders of this method are Jaenisch, Mintz etc. Gordon and Palmiter [1] successively obtained transgenic animals by this method. This method is commonly used at present, and now the transgenic animal research is mostly going based on the Palmiter method, or the method on its improvement. Wang Minhua [2] reported he produced transgenic rabbits with microinjection method transferring anti plague virus nucleic acid enzyme gene. Application of Krimpenfort produced transgenic bovine using both embryos cultured in vitro and microinjection methods.