Study of Potential Application of Cyanobacteria Leptolyngbya Valderiana in Skin Cosmeceuticals

INTERNATIONAL JOURNAL OF APPLIED PHARMACEUTICAL SCIENCES AND RESEARCH Pub Date : 2021-10-01 DOI:10.21477/ijapsr.6.4.01

Shraddha Ratnaparkhe, D. Mali, B. Nabar

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引用次数: 1

Abstract

Skin is the outermost cover and first line of defense of the human body. Skin cosmeceuticals are therapeutic agents used to maintain flawless skin and prevent skin disorders. Considering the ill effects of synthetic active agents, natural metabolites are preferred for skincare. Cyanobacterium was known to produce various secondary metabolites with therapeutic potential. This study focuses on screening whole-cell extract of Leptolyngbya valderiana for skincare. Isolation and identification of cyanobacteria were made. The whole-cell extract of Leptolyngbya valderiana was studied for its metabolite profile, mainly for proteins, carbohydrate, and lipid content. Screening of whole-cell extract was carried out for its (2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) (ABTS) radical scavenging ability and UV absorption potential. The antimicrobial effect of extracts was determined by agar cup and minimum inhibitory concentration (MIC) method using salicylic acid as a standard. Skin whitening potential was determined using tyrosinase inhibition assay. Leptolyngbya valderiana was isolated from the tree bark and selected for further study. The extract of Leptolyngbya valderiana was found to have 25.22% proteins, 36.65 % carbohydrates and 35.97% lipids. The extract was found to have antioxidant potential with 96.55% ABTS radical scavenging potential. Antimicrobial potency of the extract against Staphylococcus aureus ATCC 6538, Propionibacterium acne MTCC 1351, Candida albicans ATCC 10231 was equivalent to salicylic acid. The test extract was found to inhibit the tyrosinase enzyme by 43.46%. The test extract is rich in proteins, carbohydrates, and lipids. The extract has significant antioxidant potential, antimicrobial efficiency, ability to absorb ultraviolet B and ultraviolet A region, tyrosinase inhibition potential indicative photoprotective, and skin whitening potential. The isolate Leptolyngbya valderiana has a promising potential for skin cosmeceuticals.

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蓝细菌在皮肤药妆中的潜在应用研究

皮肤是人体最外层的保护层和第一道防线。皮肤药妆品是用来保持皮肤无瑕和预防皮肤疾病的治疗剂。考虑到合成活性剂的不良影响，天然代谢物是护肤的首选。众所周知，蓝藻可以产生各种具有治疗潜力的次级代谢物。本研究的重点是筛选细缕草全细胞提取物用于护肤。对蓝藻菌进行了分离鉴定。研究了缬草全细胞提取物的代谢产物，主要是蛋白质、碳水化合物和脂质含量。对全细胞提取物(2,2′-氮基-双(3-乙基苯并噻唑-6-磺酸))(ABTS)自由基清除能力和紫外吸收电位进行了筛选。以水杨酸为标准品，采用琼脂杯法和最低抑菌浓度法测定提取物的抑菌效果。用酪氨酸酶抑制法测定皮肤美白电位。从树皮中分离得到缬草细麻，并对其进行了进一步的研究。结果表明，缬草提取物的蛋白质含量为25.22%，碳水化合物含量为36.65%，脂质含量为35.97%。结果表明，该提取物具有抗氧化能力，对ABTS自由基的清除能力为96.55%。提取物对金黄色葡萄球菌ATCC 6538、痤疮丙酸杆菌MTCC 1351、白色念珠菌ATCC 10231的抑菌效果与水杨酸相当。试验提取物对酪氨酸酶的抑制作用为43.46%。试验提取物富含蛋白质、碳水化合物和脂质。该提取物具有显著的抗氧化潜力、抗菌效率、吸收紫外线B区和紫外线A区的能力、酪氨酸酶抑制潜力、指示性光保护和皮肤美白潜力。分离得到的缬草细分枝杆菌在皮肤药妆领域具有广阔的应用前景。

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INTERNATIONAL JOURNAL OF APPLIED PHARMACEUTICAL SCIENCES AND RESEARCH

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