{"title":"Callus and Shoot Regeneration from Protoplasts of Proso Millet (Panicum miliaceum L.)","authors":"James W. Heyser","doi":"10.1016/S0044-328X(84)80034-3","DOIUrl":null,"url":null,"abstract":"<div><p>A system for the regeneration of callus and albino plantlets from protoplasts of proso millet is described. Rapidly growing albino suspensions derived from mature seeds were used as the source of protoplasts. Protoplasts were cultured in drops of 0.07–0.25 ml in KM medium containing 1.0 mg · l<sup>−1</sup> 2,4-D and BAP. After 5 days, 7% of protoplasts had reformed cell walls and divided to form microcalli, but only 0.4% of plated protoplasts formed macrocalli. Over 20 albino shoots, 5 plantlets, and many roots were initiated by apparent somatic embryogenesis from protoplast-derived calli cultured for one or more passages on LS medium minus hormones.</p></div>","PeriodicalId":23797,"journal":{"name":"Zeitschrift für Pflanzenphysiologie","volume":"113 4","pages":"Pages 293-299"},"PeriodicalIF":0.0000,"publicationDate":"1984-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0044-328X(84)80034-3","citationCount":"43","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift für Pflanzenphysiologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0044328X84800343","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 43
Abstract
A system for the regeneration of callus and albino plantlets from protoplasts of proso millet is described. Rapidly growing albino suspensions derived from mature seeds were used as the source of protoplasts. Protoplasts were cultured in drops of 0.07–0.25 ml in KM medium containing 1.0 mg · l−1 2,4-D and BAP. After 5 days, 7% of protoplasts had reformed cell walls and divided to form microcalli, but only 0.4% of plated protoplasts formed macrocalli. Over 20 albino shoots, 5 plantlets, and many roots were initiated by apparent somatic embryogenesis from protoplast-derived calli cultured for one or more passages on LS medium minus hormones.
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