LC-MS Analysis and Antioxidant Activity of the Hydro-alcoholic Extract of Melissa Officinalis L. From Algeria

IF 0.5 Q4 CHEMISTRY, MULTIDISCIPLINARY

Chemistry Journal of Moldova Pub Date : 2020-12-01 DOI:10.19261/cjm.2020.778

B. Aicha, R. Rouabhi, S. Gasmi, C. Bensouici, H. Mohammedi, Imad Mennai

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引用次数: 1

Abstract

The present work focuses on evaluation of the chemical composition and antioxidant activity of the hydro-methanolic extract of Melissa officinalis L. from Algeria. The liquid chromatography-mass spectrometry analysis allowed the identification of six compounds: caffeic acid, caftaric acid, hydroxyjasmonic acid glucoside, caftaric acid glucoside, rosmarinic acid and sagerinic acid. The in-vitro antioxidant activity of the hydro-methanolic extract was evaluated by using four different methods including: radical scavenging assay (DPPH), scavenging activity (ABTS), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing power assay. The extract exhibited a relatively strong antioxidant activity compared to the synthetic antioxidants. The highest radical scavenging activity was registered using DPPH and ABTS methods, IC50= 20.53±2.64 μg/mL and 22.50±0.67 μg/mL, respectively. These results suggest that Melissa officinalis L. could be considered a potential source of natural antioxidants with potential interest in the agrochemical and pharmaceutical industries.

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阿尔及利亚梅丽莎水醇提取物的LC-MS分析及抗氧化活性研究

本文主要研究了阿尔及利亚产梅丽莎(Melissa officinalis L.)水甲醇提取物的化学成分和抗氧化活性。液相色谱-质谱分析鉴定出6种化合物:咖啡酸、咖啡酸、羟基茉莉酸葡萄糖苷、咖啡酸葡萄糖苷、迷迭香酸和sageric酸。采用自由基清除率(DPPH)、自由基清除率(ABTS)、铜还原抗氧化能力(CUPRAC)和铁还原力(fe) 4种方法评价其体外抗氧化活性。与合成抗氧化剂相比，该提取物具有较强的抗氧化活性。DPPH法和ABTS法对自由基的清除活性最高，IC50分别为20.53±2.64 μg/mL和22.50±0.67 μg/mL。这些结果表明，梅莉莎可被认为是一种潜在的天然抗氧化剂来源，具有潜在的农业化学和制药工业的兴趣。

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来源期刊

Chemistry Journal of Moldova CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

0.90

自引率

20.00%

发文量

审稿时长

12 weeks

期刊介绍： "Chemistry Journal of Moldova. General, Industrial and Ecological Chemistry" seeks to publish experimental or theoretical research results of outstanding significance and timeliness in all fields of Chemistry, including Industrial and Ecological Chemistry. The main goal of this edition is strengthening the Chemical Society of Moldova, following development of research in Moldovan chemical institutions and promotion of their collaboration with international chemical community. Manuscripts are welcome from all countries.