Cyclin D1 and PRAME expression in distinguishing melanoma in situ from benign melanocytic proliferation of the nail unit.

IF 1.1 4区环境科学与生态学 Q4 ECOLOGY

Population Ecology Pub Date : 2022-04-28 DOI:10.1186/s13000-022-01218-3

Young Jae Kim, Chang Jin Jung, Hyoungmin Na, Woo Jin Lee, Sung Eun Chang, Mi Woo Lee, Chan-Sik Park, Youngkyoung Lim, Chong Hyun Won

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引用次数: 8

Abstract

Background: Distinguishing benign lesion from early malignancy in melanocytic lesions of the nail unit still remains a diagnostic challenge, both clinically and histopathologically. While several immunohistochemistry (IHC) stainings have been suggested to help discriminate benign subungual melanocytic proliferation (SMP) and subungual melanoma in situ (MIS), the diagnostic utility of IHC staining for cyclin D1 and PRAME has not been thoroughly investigated in melanocytic lesions of nail unit.

Methods: This retrospective study included cases of benign SMP and subungual MIS confirmed by biopsy at Asan Medical Center from January 2016 to December 2020. Cases of melanocytic activation without proliferation and melanoma where dermal invasion was identified were excluded. Cyclin D1 and PRAME expression was assessed by counting proportion of melanocytes with nuclear positivity under 200x magnification.

Results: A total of 14 patients with benign SMP and 13 patients with subungual MIS were included in this study. 11 patients with benign SMP (71.4%) and 5 patients with subungual MIS (38.5%) showed > 60% nuclear immunostaining for cyclin D1, respectively. While 13 patients with benign SMP (92.9%) showed totally negative staining for PRAME, 10 patients with subungual MIS (76.9%) exhibited > 50% nuclear immunostaining for PRAME. Using the cutoff of 10%, PRAME exhibited good overall discrimination between benign SMP and subungual MIS (AUC = 0.849, 95% CI = 0.659-0.957).

Conclusions: This study suggests that PRAME IHC staining as a reliable discriminator in distinguishing subungual MIS from benign SMP.

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细胞周期蛋白 D1 和 PRAME 表达在区分原位黑色素瘤和指甲良性黑色素细胞增生中的作用。

背景：从临床和组织病理学角度来看，如何区分指甲黑色素细胞病变中的良性病变和早期恶性病变仍然是一个诊断难题。虽然有几种免疫组化（IHC）染色被认为有助于区分良性甲下黑色素细胞增生（SMP）和甲下原位黑色素瘤（MIS），但细胞周期蛋白 D1 和 PRAME 的 IHC 染色对甲下黑色素细胞病变的诊断作用尚未得到深入研究：这项回顾性研究纳入了2016年1月至2020年12月期间牙山医疗中心经活检证实的良性SMP和甲下MIS病例。不包括黑色素细胞活化但未增殖的病例，也不包括已发现真皮侵犯的黑色素瘤病例。在200倍放大镜下，通过计算核阳性黑色素细胞的比例来评估细胞周期蛋白D1和PRAME的表达：本研究共纳入 14 例良性 SMP 患者和 13 例真皮下 MIS 患者。其中，11 例良性 SMP 患者（71.4%）和 5 例真皮下 MIS 患者（38.5%）的细胞周期蛋白 D1 核免疫染色均大于 60%。13例良性SMP患者（92.9%）的PRAME染色完全阴性，而10例舌下MIS患者（76.9%）的PRAME核免疫染色>50%。以10%为临界值，PRAME在良性SMP和舌下MIS之间表现出良好的整体鉴别能力（AUC = 0.849, 95% CI = 0.659-0.957）：本研究表明，PRAME IHC 染色是区分舌下 MIS 和良性 SMP 的可靠鉴别指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Population Ecology 环境科学-生态学

CiteScore

3.90

自引率

11.80%

发文量

审稿时长

18-36 weeks

期刊介绍： Population Ecology, formerly known as Researches on Population Ecology launched in Dec 1952, is the official journal of the Society of Population Ecology. Population Ecology publishes original research articles and reviews (including invited reviews) on various aspects of population ecology, from the individual to the community level. Among the specific fields included are population dynamics and distribution, evolutionary ecology, ecological genetics, theoretical models, conservation biology, agroecosystem studies, and bioresource management. Manuscripts should contain new results of empirical and/or theoretical investigations concerning facts, patterns, processes, mechanisms or concepts of population ecology; those purely descriptive in nature are not suitable for this journal. All manuscripts are reviewed anonymously by two or more referees, and the final editorial decision is made by the Chief Editor or an Associate Editor based on the referees'' evaluations.