Klebsiella-candida arthritis model in Wistar rats

Abstract

Objective - development of Klebsiella - Candida arthritis model in Wistar rats. Material and methods. The study was carried out on 20 male Wistar rats, of which 15 animals were injected with a bacterial-fungal suspension by introducing into the cavity of the hock (tarsus) joint Klebsiella-Candida suspension (1: 1) with a microorganism concentration of 106 CFU / ml , using museum strains Klebsiella pneumoniae ICIS - 278 and Candida albicans ATCC 24433 under the control of an X-ray apparatus FireCR +. Five intact animals served as control. On the 15th day of the experiment, after euthanasia, a bacteriological and histological examination of the affected joints was carried out while maintaining their topography. Results. In rats infected with Klebiella-candidiasis suspension, pain syndrome and functional changes typical for arthritis were noted on days 2-3. Bacteriological examination of the affected joints in 75% of rats isolated cultures of Klebsiella pneumoniae, identical, according to PCR, to the original strain, which is part of the bacterial-fungal suspension. At the same time, fungi of the genus Candida, also used in the experiment for infecting animals, were not detected from the affected joints of rats. The reproduced model of Klebsiella-Candida arthritis is characterized by pathomorphological signs of panarthritis, accompanied by purulent inflammation of all joint structures (synovial membrane, cartilage and bone tissue) with the involvement of soft periarticular tissues. Conclusions. The presented model of Klebsiella-fungal arthritis can be used to test new means of antimicrobial (antibacterial and antifungal) therapy, which are an integral component of the treatment of arthritis associated with infection.