Identification and Sequence Determination of mRNAs Detected in Dormant (Diapausing) Aedes triseriatus Mosquito Embryos

B. Blitvich, A. Rayms-keller, C. Blair, B. Beaty
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引用次数: 10

Abstract

Many insects survive adverse climatic conditions in a dormant state known as diapause. In this study, we identified and sequenced several mRNAs in diapausing Aedes triseriatus mosquito embryos. Using reverse-transcription PCR and 5′ RACE, we identified a 995-nucleotide cDNA that encodes a 259-amino acid protein of unknown function. This putative protein displays strong sequence similarity to Drosophila mela-nogaster (95%), human (87%), Caenorhabditis elegans (86%) and yeast (81%) counterparts. The second identified full-length cDNA consists of 624 nucleotides and encodes a 174-amino acid protein of unknown function. This putative protein displays significant sequence similarity to D. melanogaster (68%), human (59%), plant (57%) and yeast (49%) counterparts. We also detected a number of cDNA fragments that exhibited significant sequence similarity to a mitochondrial cytochrome C oxidase subunit, human N33 protein (a potential human prostate tumor suppressor), 18S and 28S ribosomal RNAs, protein disulfide-isomerase, and guanine nucleotide-binding protein.
休眠(滞育)三体伊蚊胚胎mrna的鉴定与序列测定
许多昆虫在被称为滞育的休眠状态下在恶劣的气候条件下存活下来。在本研究中,我们鉴定并测序了滞育三体伊蚊胚胎中的几个mrna。利用反转录PCR和5 ' RACE技术,我们鉴定出一个995个核苷酸的cDNA,编码一个功能未知的259个氨基酸的蛋白质。该推测的蛋白序列与果蝇(95%)、人类(87%)、秀丽隐杆线虫(86%)和酵母(81%)具有很强的相似性。第二个全长cDNA由624个核苷酸组成,编码一个174个氨基酸的功能未知的蛋白质。该推测的蛋白与黑胃菌(68%)、人类(59%)、植物(57%)和酵母(49%)的对应蛋白具有显著的序列相似性。我们还检测到许多cDNA片段与线粒体细胞色素C氧化酶亚基、人类N33蛋白(一种潜在的人类前列腺肿瘤抑制因子)、18S和28S核糖体rna、蛋白二硫异构酶和鸟嘌呤核苷酸结合蛋白具有显著的序列相似性。
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