Screening for production of amylase and protease by locally-isolated Bacillus spp. from soil collected in Taguig City and Clark Freeport Zone, Philippines

Q4 Pharmacology, Toxicology and Pharmaceutics

Pharmaceutical Sciences Asia Pub Date : 2022-01-01 DOI:10.29090/psa.2022.02.21.156

John Paul Matthew Guzman, Sheila Mantaring, E. Panerio

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引用次数: 1

Abstract

Amylase and protease are two of the most commonly used enzymes in the pharmaceutical industry. Particularly, Bacillus spp. is widely regarded as a “factory” of these enzymes. In this study, a total of 40 isolates were collected from four soil samples from different metropolitan sites namely, near Chemicals and Energy Division (CED), and National Metrology Laboratory (NML) oil tankers parking lot inside the Department of Science and Technology (DOST) Compound in Taguig City, and two metropolitan-volcanic sites in Clark Freeport Zone, Pampanga (CRK1, CRK2). Of these, 33 isolates were deemed putative Bacillus spp. via phenotypic assays and were screened for the production of amylase and protease. Results showed that 26 of the screened isolates were able to produce protease and 12 were positive for amylase production. Molecular identification revealed that the enzyme-producing isolates were Bacillus spp., B. cereus, B. aryabhattai, B. amyloliquefaciens, B. firmus , B. velezensis , and Fictibacillus sp. No isolate was able to produce amylase alone. These results show the potential of Bacillus spp. from metropolitan soil as sources of pharmaceutically-important enzymes.

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菲律宾塔吉格市和克拉克自由港地区土壤中分离的芽孢杆菌产淀粉酶和蛋白酶的筛选

淀粉酶和蛋白酶是制药工业中最常用的两种酶。特别是，芽孢杆菌被广泛认为是这些酶的“工厂”。在本研究中，共从4个不同的大都市站点的土壤样本中收集了40个分离株，这些站点分别是大庆市科技部(DOST)大院内化学和能源部门(CED)和国家计量实验室(NML)油轮停车场附近，以及邦板卡克拉克自由港区(CRK1, CRK2)的两个大都市-火山站点。其中33株菌株经表型鉴定为芽孢杆菌，并进行淀粉酶和蛋白酶的筛选。结果表明，筛选的分离株中有26株能产生蛋白酶，12株淀粉酶产生阳性。分子鉴定结果表明，产酶菌株为芽孢杆菌、蜡样芽孢杆菌、aryabhattai芽孢杆菌、解淀粉芽孢杆菌、B. firmus、B. velezensis和Fictibacillus sp.。这些结果表明，从都市土壤中提取的芽孢杆菌具有作为重要药用酶来源的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmaceutical Sciences Asia Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The Pharmaceutical Sciences Asia (PSA) journal is a double-blinded peer-reviewed journal in English published quarterly, by the Faculty of Pharmacy, Mahidol University, Thailand. The PSA journal is formerly known as Mahidol University Journal of Pharmaceutical Sciences and committed to the timely publication of innovative articles and reviews. This journal is available in both printed and electronic formats. The PSA journal aims at establishing a publishing house that is open to all. It aims to disseminate knowledge; provide a learned reference in the field; and establish channels of communication between academic and research expert, policy makers and executives in industry and investment institutions. The journal publishes research articles, review articles, and scientific commentaries on all aspects of the pharmaceutical sciences and multidisciplinary field in health professions and medicine. More specifically, the journal publishes research on all areas of pharmaceutical sciences and related disciplines: Clinical Pharmacy Drug Synthesis and Discovery Targeted-Drug Delivery Pharmaceutics Biopharmaceutical Sciences Phytopharmaceutical Sciences Pharmacology and Toxicology Pharmaceutical Chemistry Nutraceuticals and Functional Foods Natural Products Social, Economic, and Administrative Pharmacy Clinical Drug Evaluation and Drug Policy Making Antimicrobials, Resistance and Infection Control Pharmacokinetics and Pharmacodynamics.