Freshwater Actinobacteria from sediments of the deep and ancient Lake Baikal (Russia) and their genetic potential as producers of secondary metabolites

IF 1.6 4区 环境科学与生态学 Q3 ECOLOGY
E. Protasov, D. Axenov-Gribanov, Z. Shatilina, M. Timofeyev, A. Lane
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引用次数: 2

Abstract

Actinobacteria from terrestrial and marine environments produce a variety of natural products that mediate interand intraspecies interactions. In contrast, the potential of freshwater Actinobacteria for secondary metabolite production remains underexplored. Large lakes with a long evolutionary history might contain microflora subjected to unique environmental conditions that favor the evolution of unique metabolic capabilities. One such lake is Lake Baikal (Russia), the deepest lake on earth as well as one of the oldest. In this study, we investigated the genetically encoded secondary metabolic potential of 24 Actinobacteria strains isolated from Lake Baikal sediments. PCR-based screening for genes encoding type I and type II polyketide synthases (PKSs), nonribosomal peptide synthetases (NRPSs), and halogenases confirmed that all strains possessed at least 1 of these biosynthetic genes. Both PKSs and NRPSs were widely distributed, while halogenase-encoding genes were not detected. Phylogenetic comparison of type I PKS ketosynthase (KS) domain sequences between freshwater isolates and marine and terrestrial strains supported overlap between KSs from these 3 groups. Evaluation of antibiotic activity for chemical extracts from all isolates revealed that 75% produced metabolites inhibitory toward model bacteria and/or fungi. To our knowledge, this study is among the first evaluations of the genetically encoded secondary metabolic capabilities of freshwater sediment Actinobacteria. Our findings highlight the similarities and differences between freshwater and marine Actinobacteria secondary metabolism, suggesting the potential of freshwater Actinobacteria for the production of natural products that may play roles as mediators of interactions between organisms in freshwater habitats.
俄罗斯贝加尔湖深古沉积物中的淡水放线菌及其作为次生代谢物生产者的遗传潜力
来自陆地和海洋环境的放线菌产生多种介导种间和种内相互作用的天然产物。相比之下,淡水放线菌生产次生代谢物的潜力仍未得到充分开发。具有悠久进化历史的大型湖泊可能包含受独特环境条件影响的微生物群,这些环境条件有利于独特代谢能力的进化。其中一个湖泊是贝加尔湖(俄罗斯),它是地球上最深的湖泊,也是最古老的湖泊之一。在这项研究中,我们研究了从贝加尔湖沉积物中分离的24株放线菌的遗传编码次级代谢势。基于pcr的ⅰ型和ⅱ型聚酮合成酶(pks)、非核糖体肽合成酶(NRPSs)和卤化酶编码基因筛选证实,所有菌株至少具有1种这些生物合成基因。pks和NRPSs分布广泛,而卤化酶编码基因未检出。淡水菌株与海洋和陆地菌株的I型PKS酮合成酶(KS)结构域序列的系统发育比较表明,这3组菌株的KS存在重叠。所有分离物的化学提取物的抗生素活性评估显示,75%的分离物产生对模式细菌和/或真菌有抑制作用的代谢物。据我们所知,这项研究是对淡水沉积物放线菌遗传编码的次级代谢能力的首次评估之一。我们的研究结果强调了淡水放线菌和海洋放线菌次生代谢的异同,表明淡水放线菌在生产天然产物方面的潜力,这些天然产物可能在淡水栖息地的生物之间的相互作用中发挥中介作用。
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来源期刊
Aquatic Microbial Ecology
Aquatic Microbial Ecology 环境科学-海洋与淡水生物学
CiteScore
3.30
自引率
0.00%
发文量
8
审稿时长
3.0 months
期刊介绍: AME is international and interdisciplinary. It presents rigorously refereed and carefully selected Research Articles, Reviews and Notes, as well as Comments/Reply Comments (for details see AME 27:209), Opinion Pieces (previously called ''As I See It'') and AME Specials. For details consult the Guidelines for Authors. Papers may be concerned with: Tolerances and responses of microorganisms to variations in abiotic and biotic components of their environment; microbial life under extreme environmental conditions (climate, temperature, pressure, osmolarity, redox, etc.). Role of aquatic microorganisms in the production, transformation and decomposition of organic matter; flow patterns of energy and matter as these pass through microorganisms; population dynamics; trophic interrelationships; modelling, both theoretical and via computer simulation, of individual microorganisms and microbial populations; biodiversity. Absorption and transformation of inorganic material; synthesis and transformation of organic material (autotrophic and heterotrophic); non-genetic and genetic adaptation; behaviour; molecular microbial ecology; symbioses.
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