Characterization of GBV-CHGV viral genome: comparison among different isolates for a ∼ 2 kb-sequence that covers entire E1 and most of 5′UTR and E2

K. Takahashi , M. Hijikata , K. Aoyama , H. Hoshino , K. Hino , S. Mishiro
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引用次数: 20

Abstract

It is yet not fully uncovered where and how translation starts in GB virus C or hepatitis G virus (GBV-CHGV), and, accordingly, information on structural proteins of this virus has been scarce. To know a vertebral architecture of GBV-CHGV genome at least for its 5′ portion, we analyzed 31 Japanese isolates of GBV-CHGV. Our results indicated that most of the Japanese isolates were classifiable into ‘Japanese type’, two isolates into ‘North American type’, one into ‘African type’, and another into the fourth distinct type. Despite such sequence diversity across individual types, however, several common characteristics existed. (1) The AUG that resides just downstream of an oligopyrimidine tract was conserved by all isolates as the first in-frame initiation codon for the polyprotein. (2) The sequence upstream of this AUG had many covariant nucleotide substitutions, suggesting conserved secondary structure. (3) The ORF that starts with this AUG did not code for core. (4) Predicted amino acid sequence of putative E1 and E2 was highly conserved among different types, and did not contain hypervariable regions.

GBV-CHGV病毒基因组的表征:覆盖整个E1和大部分5'UTR和E2的~ 2 kb序列的不同分离株的比较
目前还没有完全揭示GB病毒C或G型肝炎病毒(GBV-CHGV)的翻译在哪里以及如何开始,因此,关于该病毒结构蛋白的信息很少。为了了解GBV-CHGV基因组至少5 '部分的椎体结构,我们分析了31株日本GBV-CHGV分离株。结果表明,大多数日本分离株可分为“日本型”,2株为“北美型”,1株为“非洲型”,1株为第四型。然而,尽管个体类型之间存在这种序列多样性,但存在一些共同特征。(1)位于寡聚嘧啶通道下游的AUG作为多蛋白的第一个框架内起始密码子在所有分离物中都被保守。(2)该AUG上游序列存在多个协变核苷酸取代,二级结构较为保守。(3)以这个AUG开头的ORF没有为core编码。(4)推测的E1和E2氨基酸序列在不同类型间具有高度保守性,不存在高变区。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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