Impact of Gibberellic Acid and Naphthalene Acetic Acid on Axillary Shoot Multiplication in Hygrophila polysperma (Roxb.) T. Anderson

Q1 Engineering

Periodicals of Engineering and Natural Sciences Pub Date : 2022-12-26 DOI:10.28978/nesciences.1224554

M. Doğan

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引用次数: 0

Abstract

In this work, axillary shoot regenerations were investigated from nodal explants of Hygrophila polysperma (Roxb.) T. Anderson in MS medium (Murashige and Skoog) including different combinations of Gibberellic Acid (GA3) and Naphthalene Acetic Acid (NAA). Nodal explants of H. polysperma were used in propagation studies. The 100% regeneration frequency was determined in MS plus 0.25 and 0.50 mg/L GA3 + 0.15 mg/L NAA. When the shoot numbers were examined, the best nutrient medium was determined as 0.25 mg/L GA3 + 0.15 mg/L NAA (14.72 shoots/explant). The best result in shoot length was reached in MS plus 0.50 mg/L GA3 + 0.15 mg/L NAA (1.91 cm). Then the longest shoot was determined as 1.79 cm in nutrient media plus 0.25 mg/L GA3 + 0.15 mg/L NAA. Longer shoots were obtained in GA3+NAA nutrient media according to control. When the results were examined, it was determined that the number and length of shoots decreased as the amount of GA3+NAA used in the culture medium increased. Regenerated shoots were rooted in MS media including 0.25 mg/L Indole-3-Acetic Acid and successfully acclimatized to aquarium conditions. As a result, multiple and rapid productions of H. polysperma under tissue culture conditions were achieved.

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赤霉素酸和萘乙酸对喜水多精植物腋芽增殖的影响t·安德森

本研究以嗜水多精菌(Roxb.)结状外植体为材料，对其腋芽再生进行了研究。MS培养基(Murashige和Skoog)，包括赤霉素酸(GA3)和萘乙酸(NAA)的不同组合。用多精草结状外植体进行了繁殖研究。在MS + 0.25和0.50 mg/L GA3 + 0.15 mg/L NAA条件下测定100%再生频率。测定芽数时，最佳培养基为0.25 mg/L GA3 + 0.15 mg/L NAA(14.72个芽/外植体)。茎长以MS + 0.50 mg/L GA3 + 0.15 mg/L NAA (1.91 cm)处理效果最佳。在营养培养基+ 0.25 mg/L GA3 + 0.15 mg/L NAA条件下，最长芽为1.79 cm。对照在GA3+NAA营养培养基上可获得较长的芽。结果表明，随着培养基中GA3+NAA用量的增加，芽数和芽长均减少。再生芽在含0.25 mg/L吲哚-3-乙酸的MS培养基上生根，成功地适应了水缸条件。结果表明，在组织培养条件下，可以实现多精精子的快速繁殖。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Periodicals of Engineering and Natural Sciences Engineering-Architecture

CiteScore

1.90

自引率

0.00%

发文量

140

审稿时长

7 weeks

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