Effect of delmopinol application on Candida albicans adherence on denture-bearing surface of heat-cured and cold-cured acrylic resins – An in vitro study

Abstract

Purpose: In this in vitro study, we evaluate the effect of delmopinol application on Candida albicans adherence on heat-cured acrylic resin and cold-cured acrylic resin. Materials and Methods: A total of 60 specimens of every sort of acrylic resin were made, 20 specimens of each type were control group, 20 specimens were contaminated before delmopinol application, and 20 specimens were contaminated after delmopinol application. Each specimen in each tube was individually transferred to a spectrophotometer at 530 nm wavelength to measure the turbidity degree through the transmittance. Aliquots of 10 μl of every tube were then collected and inoculated into agar Sabouraud plates containing 500 mmol/l of sucrose, which was incubated for 24 h at 37°C, so as to see the microbial growth. Two-way ANOVA analysis of variance test and post hoc Turkey's test were carried out to ascertain the level of significance (P < 0.001) of various observations. Conclusion: In case of contamination after delmopinol application, heat-cured acrylic resin showed a greater reduction in the adherence of C. albicans in this study.