Fabrication and application of nanofork for measuring single cells adhesion force inside ESEM

M. Ahmad, M. Nakajima, M. Kojima, S. Kojima, M. Homma, T. Fukuda
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Abstract

Cell-substrate adhesion and cell-cell adhesion are very important in promoting other cell activities such as cell proliferation and cell differentiation [1]. Furthermore, the knowledge of the cell adhesion force can be used in various applications such as in the determination of the cell viability and the evaluation of the quality of the artificial scaffold. Although there are several force measurement techniques available, e.g. atomic force microscopy (AFM) cantilever [2], optical tweezer [3] and micropipette, these techniques are difficult to be implemented directly when dealing with the cell adhesion measurement.
纳米叉子在ESEM内单细胞粘附力测量中的制备与应用
细胞-底物粘附和细胞-细胞粘附在促进细胞增殖和细胞分化等其他细胞活动中非常重要[1]。此外,细胞粘附力的知识可用于各种应用,如细胞活力的测定和人工支架质量的评估。虽然有几种力测量技术可用,如原子力显微镜(AFM)悬臂[2],光学镊子[3]和微移液,但这些技术在处理细胞粘附测量时难以直接实施。
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