Arı Spermasının Dondurma-Çözdürme Sonrası Spermatolojik Parametreleri Üzerine Metiyonin ve Sisteaminin Etkileri

4区农林科学 Q4 Veterinary

International Journal of Applied Research in Veterinary Medicine Pub Date : 2021-11-11 DOI:10.30782/jrvm.991436

Selim Alçay, Selvinar Seven-Çakmak, Ahmet Aktar, Ibrahim Cakmak

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Abstract

sperma and (2.5 (P<0.05). Abstract In this study, it was aimed to determine the effects of methionine and cysteamine in the cryopreservation process of drone semen (Apis mellif-era). Semen samples were collected from sexually mature drones and pooled. The pooled semen was then divided into five equal volumes and diluted with different concentrations of methionine (2.5 and 5 mM), cysteamine (2.5 and 5 mM) and control. Post-thawed motility values; Methionine5, Cysteamine2,5 and Cysteamine5 groups had a possitive effect on compared to the the control group (P<0.05). It was observed that the antioxidant groups protected the plasma membrane integrity better than the control group (P<0.05). Acrosomal integrity rates was better in the methionine groups compared to the control group (P<0.05). Membrane lipid peroxidation was analyzed by measuring malondialdehyde (MDA) concentration post-thawed. It was observed that antioxidant-containing extenders had beneficial effects on drone semen parameters. Considering the semen parameters; The Methionine5 group was found to be the most effective group for freezing drone semen.

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(2.5) (P<0.05)。摘要本研究旨在探讨蛋氨酸和半胱胺在蜜蜂精液冷冻保存过程中的作用。从性成熟的雄蜂中收集精液样本并汇总。将收集的精液分成5个等体积，分别用不同浓度的蛋氨酸(2.5和5mm)、半胱胺(2.5和5mm)和对照稀释。解冻后运动值;蛋氨酸5、半胱氨酸2、半胱氨酸5和半胱氨酸5组与对照组相比有显著的正相关(P<0.05)。结果表明，各抗氧化组对细胞膜完整性的保护效果优于对照组(P<0.05)。蛋氨酸组顶体完整性率高于对照组(P<0.05)。通过测定解冻后丙二醛(MDA)浓度分析膜脂过氧化作用。结果表明，含抗氧化剂的膨化剂对雄蜂精液参数有良好的影响。考虑精液参数;蛋氨酸5组是冷冻雄蜂精液最有效的组。

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来源期刊

International Journal of Applied Research in Veterinary Medicine 农林科学-兽医学

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： The International Journal of Applied Research in Veterinary Medicine promotes excellence in the clinical practice of veterinary medicine by disseminating fundamental scientific, diagnostic, and treatment knowledge gained from prospective and retrospective research in a timely manner. The Journal fulfills its mission through rapid peer review of each submitted article, and publication of all articles within 90 days of acceptance. All published articles meet the standards of Balance, Independence, Objectivity and Scientific Rigor.