Formulation preparation and evaluation of Apigenin ointment for anti-inflammatory and wound healing activities

Abstract

Objective: - Objective of research work was to prepare the formulation and evaluation of Apigenin (Ap) loaded ointment (Ont) for anti-inflammatory and wound healing activities. Ap-loaded ointment Material and Methods: was prepared by fusion method and evaluated using different parameters. The optimized ointment formulation was further used for the anti-inflammatory and wound healing activities studies. The anti-inflammatory and wound healing activities was performed on albino wistar rats by using caragineen paw edema method and wound healing activity was performed using Incision wound model. Optimized F6 formulation was found to be good Results: physical properties such as colour, odour, pH, spreadability, extrudability and diffusion method used. The diffusion of F6 was indicating a 72% drug release; and was inhibited 59.97% paw edoema when compared to the control group. Result indicated that apigenin is a bioactive anti-inflammatory molecule. It was key components for producing anti-inflammatory property. According to the results of measurement of tensile strength, hydroxyproline estimation, hexosamine and protein estimation were showed F6 topical formulation have potential wound healing activity. It may be due to its antioxidants and possible inhibitors of cyclo-oxygenase, lipoxygenase, and nitric oxide synthase action. Conclusion: - Apigenin (Ap) loaded ointment (Ont) for anti-inflammatory and wound healing activities can be used as successful drug-delivery system for wound healing.