Study of In vitro Root Induction and Hardening Responses of Four Pyrus spp.

Abstract

Explants of three P. betulifolia (Bet 1, Bet 2 and Bet3) and P. ussuriensis (Uss) were tested for root induction in vitro and hardening in vivo. Bet 1 was the best rapid rooting response among four pears. The maximum rooting percent and the highest number of roots were 86.67% and 7.33 respectively that were observed in Bet 1 at ½MS basal medium supplemented with 0.3 mg/l IBA and quick-dip 15s of 2 mg IBA. Other Bet 2 and 3 were not different with each other. But Uss was produced the longest root length. It was 3.23cm at ½MS basal medium containing 0.2 mg/l IBA and 15s quick-dip of 2mg IBA. Almost 60 % of Bet 1 explants were hardened.